Статья

Transcriptome Analysis of Taxillusi chinensis (DC.) Danser Seeds in Response to Water Loss

Shugen WeiGuangxi Botanical Garden of Medicinal Plants, Nanning, Guangxi, ChinaXiaojun MaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, ChinaLimei PanGuangxi Botanical Garden of Medicinal Plants, Nanning, Guangxi, ChinaJianhua MiaoGuangxi Botanical Garden of Medicinal Plants, Nanning, Guangxi, ChinaJine FuGuangxi Botanical Garden of Medicinal Plants, Nanning, Guangxi, ChinaLonghua BaiYunnan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Jinghong, ChinaZhonglian ZhangYunnan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Jinghong, ChinaYan-Hong GuanYunnan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Jinghong, ChinaChangming MoGuangxi Botanical Garden of Medicinal Plants, Nanning, Guangxi, ChinaHao HuangGuangxi Botanical Garden of Medicinal Plants, Nanning, Guangxi, ChinaMaoshan ChenDepartment of Biochemistry and Genetics, La Trobe Institute for Molecular Science (LIMS), La Trobe University, Melbourne, Victoria, Australia

2017en

ABI

Аннотация

BACKGROUND: Taxillus chinensis (DC.) Danser, the official species of parasitic loranthus that grows by parasitizing other plants, is used in various traditional Chinese medicine prescriptions. ABA-dependent and ABA-independent pathways are two major pathways in response to drought stress for plants and some genes have been reported to play a key role during the dehydration including dehydration-responsive protein RD22, late embryogenesis abundant (LEA) proteins, and various transcription factors (TFs) like MYB and WRKY. However, genes responding to dehydration are still unknown in loranthus. METHODS AND RESULTS: Initially, loranthus seeds were characterized as recalcitrant seeds. Then, biological replicates of fresh loranthus seeds (CK), and seeds after being dehydrated for 16 hours (Tac-16) and 36 hours (Tac-36) were sequenced by RNA-Seq, generating 386,542,846 high quality reads. A total of 164,546 transcripts corresponding to 114,971 genes were assembled by Trinity and annotated by mapping them to NCBI non-redundant (NR), UniProt, GO, KEGG pathway and COG databases. Transcriptome profiling identified 60,695, 56,027 and 66,389 transcripts (>1 FPKM) in CK, Tac-16 and Tac-36, respectively. Compared to CK, we obtained 2,102 up-regulated and 1,344 down-regulated transcripts in Tac-16 and 1,649 up-regulated and 2,135 down-regulated transcripts in Tac-36 by using edgeR. Among them some have been reported to function in dehydration process, such as RD22, heat shock proteins (HSP) and various TFs (MYB, WRKY and ethylene-responsive transcription factors). Interestingly, transcripts encoding ribosomal proteins peaked in Tac-16. It is indicated that HSPs and ribosomal proteins may function in early response to drought stress. Raw sequencing data can be accessed in NCBI SRA platform under the accession number SRA309567. CONCLUSIONS: This is the first time to profile transcriptome globally in loranthus seeds. Our findings provide insights into the gene regulations of loranthus seeds in response to water loss and expand our current understanding of drought tolerance and germination of seeds.

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Идентификаторы

DOI: 10.1371/journal.pone.0169177

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Цитирований: 9Использованных источников: 0

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