Статья

Chromosome-level assembly of the water buffalo genome surpasses human and goat genomes in sequence contiguity

Wai Yee LowThe Davies Research Centre, School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy, SA, 5371, AustraliaRick TearleThe Davies Research Centre, School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy, SA, 5371, AustraliaDerek M. BickhartCell Wall Biology and Utilization Laboratory, ARS USDA, Madison, WI, 53706, USABenjamin D. RosenAnimal Genomics and Improvement Laboratory, ARS USDA, Beltsville, MD, 20705, USASarah B. KinganPacific Biosciences, Menlo Park, CA, 94025, USAThomas SwaleDovetail Genomics, Santa Cruz, CA, 95060, USAFrançoise Thibaud‐NissenNational Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, 20894, USATerence D. MurphyNational Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, 20894, USARachel YoungThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, United KingdomLucas LefèvreThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, United KingdomDavid HumeMater Research Institute-University of Queensland, Translational Research Institute, Woolloongabba, Brisbane, Queensland, 4102, AustraliaAndrew CollinsGenetic Epidemiology and Bioinformatics, University of Southampton, Southampton, SO16 6YD, UKPaolo Ajmone‐MarsanDepartment of Animal Science, Food and Technology - DIANA, and Nutrigenomics and Proteomics Research Center - PRONUTRIGEN, Università Cattolica del Sacro Cuore, Via Emilia Parmense 84, 29122, Piacenza, PC, ItalyTimothy P. L. SmithUS Meat Animal Research Center, ARS USDA, Clay Center, Ne, 68933, USAJ. L. WilliamsThe Davies Research Centre, School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy, SA, 5371, Australia. [email protected]

2019en

ABI

Аннотация

Rapid innovation in sequencing technologies and improvement in assembly algorithms have enabled the creation of highly contiguous mammalian genomes. Here we report a chromosome-level assembly of the water buffalo (Bubalus bubalis) genome using single-molecule sequencing and chromatin conformation capture data. PacBio Sequel reads, with a mean length of 11.5 kb, helped to resolve repetitive elements and generate sequence contiguity. All five B. bubalis sub-metacentric chromosomes were correctly scaffolded with centromeres spanned. Although the index animal was partly inbred, 58% of the genome was haplotype-phased by FALCON-Unzip. This new reference genome improves the contig N50 of the previous short-read based buffalo assembly more than a thousand-fold and contains only 383 gaps. It surpasses the human and goat references in sequence contiguity and facilitates the annotation of hard to assemble gene clusters such as the major histocompatibility complex (MHC).

Перевод пока недоступен

Идентификаторы

DOI: 10.1038/s41467-018-08260-0

Цитирования и источники

Цитирований: 2Использованных источников: 0

Показатели — AkademScholar