Статья

Genome‐wide association study discovered candidate genes of Verticillium wilt resistance in upland cotton (<i>Gossypium hirsutum</i> L.)

Tinggang LiLaboratory of Cotton Disease Institute of Food Science and Technology Chinese Academy of Agricultural Sciences Beijing ChinaXuefeng MaLaboratory of Cotton Disease Institute of Food Science and Technology Chinese Academy of Agricultural Sciences Beijing ChinaNanyang LiLaboratory of Cotton Disease Institute of Food Science and Technology Chinese Academy of Agricultural Sciences Beijing ChinaLei ZhouLaboratory of Cotton Disease Institute of Food Science and Technology Chinese Academy of Agricultural Sciences Beijing ChinaLiu ZhengXinjiang Academy of Agricultural and Reclamation Science Xinjiang ChinaHuanyong HanXinjiang Academy of Agricultural and Reclamation Science Xinjiang ChinaYuejing GuiLaboratory of Cotton Disease Institute of Food Science and Technology Chinese Academy of Agricultural Sciences Beijing ChinaYuming BaoLaboratory of Cotton Disease Institute of Food Science and Technology Chinese Academy of Agricultural Sciences Beijing ChinaJieyin ChenLaboratory of Cotton Disease Institute of Food Science and Technology Chinese Academy of Agricultural Sciences Beijing ChinaXiaofeng DaiLaboratory of Cotton Disease Institute of Food Science and Technology Chinese Academy of Agricultural Sciences Beijing China

2017en

ABI

Аннотация

Summary Verticillium wilt ( VW ), caused by infection by Verticillium dahliae , is considered one of the most yield‐limiting diseases in cotton. To examine the genetic architecture of cotton VW resistance, we performed a genome‐wide association study ( GWAS ) using a panel of 299 accessions and 85 630 single nucleotide polymorphisms ( SNP s) detected using the specific‐locus amplified fragment sequencing ( SLAF ‐seq) approach. Trait– SNP association analysis detected a total of 17 significant SNP s at P < 1.17 × 10 –5 ( P = 1/85 630, –log 10 P = 4.93); the peaks of SNP s associated with VW resistance on A10 were continuous and common in three environments ( RDIG 2015, RDIF 2015 and RDIF 2016). Haplotype block structure analysis predicted 22 candidate genes for VW resistance based on A10_99672586 with a minimum P ‐value (–log 10 P = 6.21). One of these genes ( CG 02) was near the significant SNP A10_99672586 (0.26 Mb), located in a 372‐kb haplotype block, and its Arabidopsis AT 3G25510 homologues contain TIR ‐ NBS ‐ LRR domains that may be involved in disease resistance response. Real‐time quantitative PCR and virus‐induced gene silencing ( VIGS ) analysis showed that CG 02 was specific to up‐regulation in the resistant (R) genotype Zhongzhimian2 ( ZZM 2) and that silenced plants were more susceptible to V. dahliae . These results indicate that CG 02 is likely the candidate gene for resistance against V. dahliae in cotton. The identified locus or gene may serve as a promising target for genetic engineering and selection for improving resistance to VW in cotton.

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Идентификаторы

DOI: 10.1111/pbi.12734

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