Статья

Reference genome sequences of two cultivated allotetraploid cottons, Gossypium hirsutum and Gossypium barbadense

Maojun WangNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaLili TuNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaDaojun YuanCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaDe ZhuNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaChao ShenNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaJianying LiNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaFuyan LiuLiuling PeiNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaPengcheng WangNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaGuannan ZhaoNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaZhengxiu YeNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaHui HuangNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaFeilin YanNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaYizan MaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaLin ZhangNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaMin LiuJiaqi YouNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaYicheng YangNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaZhenping LiuNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaFan HuangNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaBaoqi LiNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaPing QiuNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaQinghua ZhangNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaLongfu ZhuNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaShuangxia JinNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaXiyan YangNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaLing MinCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaGuoliang LiHubei Key Laboratory of Agricultural Bioinformatics, College of Informatics, Huazhong Agricultural University, Wuhan, ChinaLing‐Ling ChenHubei Key Laboratory of Agricultural Bioinformatics, College of Informatics, Huazhong Agricultural University, Wuhan, ChinaHongkun ZhengKeith LindseyDepartment of Biosciences, Durham University, Durham, UK. [email protected]Zhongxu LinCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China. [email protected]Joshua A. UdallDepartment of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, IA, USA. [email protected]Xianlong ZhangNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China. [email protected]

2018en

ABI

Аннотация

Allotetraploid cotton species (Gossypium hirsutum and Gossypium barbadense) have long been cultivated worldwide for natural renewable textile fibers. The draft genome sequences of both species are available but they are highly fragmented and incomplete1–4. Here we report reference-grade genome assemblies and annotations for G. hirsutum accession Texas Marker-1 (TM-1) and G. barbadense accession 3–79 by integrating single-molecule real-time sequencing, BioNano optical mapping and high-throughput chromosome conformation capture techniques. Compared with previous assembled draft genomes1,3, these genome sequences show considerable improvements in contiguity and completeness for regions with high content of repeats such as centromeres. Comparative genomics analyses identify extensive structural variations that probably occurred after polyploidization, highlighted by large paracentric/pericentric inversions in 14 chromosomes. We constructed an introgression line population to introduce favorable chromosome segments from G. barbadense to G. hirsutum, allowing us to identify 13 quantitative trait loci associated with superior fiber quality. These resources will accelerate evolutionary and functional genomic studies in cotton and inform future breeding programs for fiber improvement. The improved genome assemblies of allotetraploid cotton species Gossypium hirsutum and Gossypium barbadense provide insights into cotton evolution and inform the construction of introgression lines used to identify loci associated with fiber quality.

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Идентификаторы

DOI: 10.1038/s41588-018-0282-x

Цитирования и источники

Цитирований: 3Использованных источников: 0

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