Статья

<i>Eg</i>MYB2, a new transcriptional activator from <i>Eucalyptus</i> xylem, regulates secondary cell wall formation and lignin biosynthesis

Monica GoicoecheaInstitut Fédératif de Recherches FR40, Unité mixte de Recherches 5546, Université Paul Sabatier-Centre National de la Recherche Scientifique, Pôle de Biotechnologie Végétale, 24 chemin de Borde Rouge, BP 17 Auzeville, 31326 Castanet-Tolosan, FranceÉric LacombeBoth authors contributed equally to this workSylvain LegayInstitut Fédératif de Recherches FR40, Unité mixte de Recherches 5546, Université Paul Sabatier–Centre National de la Recherche Scientifique, Pôle de Biotechnologie Végétale, 24 chemin de Borde Rouge, BP 17 Auzeville, 31326 Castanet-Tolosan, FranceSnježana MihaljevićInstitut Fédératif de Recherches FR40, Unité mixte de Recherches 5546, Université Paul Sabatier–Centre National de la Recherche Scientifique, Pôle de Biotechnologie Végétale, 24 chemin de Borde Rouge, BP 17 Auzeville, 31326 Castanet-Tolosan, FrancePhilippe RechInstitut Fédératif de Recherches FR40, Unité mixte de Recherches 5546, Université Paul Sabatier–Centre National de la Recherche Scientifique, Pôle de Biotechnologie Végétale, 24 chemin de Borde Rouge, BP 17 Auzeville, 31326 Castanet-Tolosan, FranceAlain JauneauInstitut Fédératif de Recherches FR40, Unité mixte de Recherches 5546, Université Paul Sabatier–Centre National de la Recherche Scientifique, Pôle de Biotechnologie Végétale, 24 chemin de Borde Rouge, BP 17 Auzeville, 31326 Castanet-Tolosan, FranceCatherine C. LapierreLaboratoire de Chimie Biologique, Unité mixte de Recherches 206, Institut National de la Recherche Agronomique-Institut National Agronomique, 78850 Thiverval-Grignon, FranceBrigitte PolletLaboratoire de Chimie Biologique, Unité mixte de Recherches 206, Institut National de la Recherche Agronomique-Institut National Agronomique, 78850 Thiverval-Grignon, FranceDaniel VerhaegenCIRAD Forêt, TA 10/C Baillarguet BP 5035, 34398 Montpellier Cédex 05, FranceNicole Chaubet‐GigotInstitut Fédératif de Recherches FR40, Unité mixte de Recherches 5546, Université Paul Sabatier–Centre National de la Recherche Scientifique, Pôle de Biotechnologie Végétale, 24 chemin de Borde Rouge, BP 17 Auzeville, 31326 Castanet-Tolosan, FranceJacqueline Grima‐PettenatiInstitut Fédératif de Recherches FR40, Unité mixte de Recherches 5546, Université Paul Sabatier–Centre National de la Recherche Scientifique, Pôle de Biotechnologie Végétale, 24 chemin de Borde Rouge, BP 17 Auzeville, 31326 Castanet-Tolosan, France

2005en

ABI

Аннотация

Summary EgMYB2, a member of a new subgroup of the R2R3 MYB family of transcription factors, was cloned from a library consisting of RNA from differentiating Eucalyptus xylem. EgMYB2 maps to a unique locus on the Eucalyptus grandis linkage map and co-localizes with a quantitative trait locus (QTL) for lignin content. Recombinant EgMYB2 protein was able to bind specifically the cis-regulatory regions of the promoters of two lignin biosynthetic genes, cinnamoyl-coenzyme A reductase (CCR) and cinnamyl alcohol dehydrogenase (CAD), which contain MYB consensus binding sites. EgMYB2 was also able to regulate their transcription in both transient and stable expression assays. Transgenic tobacco plants over-expressing EgMYB2 displayed phenotypic changes relative to wild-type plants, among which were a dramatic increase in secondary cell wall thickness, and an alteration of the lignin profiles. Transcript abundance of genes encoding enzymes specific to lignin biosynthesis was increased to varying extents according to the position of individual genes in the pathway, whereas core phenylpropanoid genes were not significantly affected. Together these results suggest a role for EgMYB2 in the co-ordinated control of genes belonging to the monolignol-specific pathway, and therefore in the biosynthesis of lignin and the regulation of secondary cell wall formation.

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Идентификаторы

DOI: 10.1111/j.1365-313x.2005.02480.x

Цитирования и источники

Цитирований: 2Использованных источников: 0

Показатели — AkademScholar