Статья

Expanded 3D Nanofiber Scaffolds: Cell Penetration, Neovascularization, and Host Response

Jiang JiangDepartment of Surgery‐Transplant and Mary & Dick Holland Regenerative Medicine Program University of Nebraska Medical Center Omaha NE 68198 USAZhuoran LiDepartment of Mechanical and Materials Engineering University of Nebraska‐Lincoln Lincoln NE 68588 USAHongjun WangDepartment of Surgery‐Transplant and Mary & Dick Holland Regenerative Medicine Program University of Nebraska Medical Center Omaha NE 68198 USAYue WangDepartment of Surgery‐Transplant and Mary & Dick Holland Regenerative Medicine Program University of Nebraska Medical Center Omaha NE 68198 USAMark A. CarlsonDepartment of Surgery VA Nebraska–Western Iowa Health Care System Omaha NE 68105 USAMatthew J. TeusinkDepartment of Orthopedic Surgery and Rehabilitation University of Nebraska Medical Center Omaha NE 68198 USAMatthew R. MacEwanDepartment of Neurosurgery Washington University School of Medicine Saint Louis MO 63110 USALinxia GuDepartment of Mechanical and Materials Engineering University of Nebraska‐Lincoln Lincoln NE 68588 USAJingwei XieDepartment of Surgery‐Transplant and Mary & Dick Holland Regenerative Medicine Program University of Nebraska Medical Center Omaha NE 68198 USA

2016en

ABI

Аннотация

Herein, a robust method to fabricate expanded nanofiber scaffolds with controlled size and thickness using a customized mold during the modified gas‐foaming process is reported. The expansion of nanofiber membranes is also simulated using a computational fluid model. Expanded nanofiber scaffolds implanted subcutaneously in rats show cellular infiltration, whereas non‐expanded scaffolds only have surface cellular attachment. Compared to unexpanded nanofiber scaffolds, more CD68 + and CD163 + cells are observed within expanded scaffolds at all tested time points post‐implantation. More CCR7 + cells appear within expanded scaffolds at week 8 post‐implantation. In addition, new blood vessels are present within the expanded scaffolds at week 2. The formed multinucleated giant cells within expanded scaffolds are heterogeneous expressing CD68, CCR7, or CD163 markers. Together, the present study demonstrates that the expanded nanofiber scaffolds promote cellular infiltration/tissue integration, a regenerative response, and neovascularization after subcutaneous implantation in rats. The use of expanded electrospun nanofiber scaffolds offers a promising method for in situ tissue repair/regeneration and generation of 3D tissue models/constructs.

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Идентификаторы

DOI: 10.1002/adhm.201600808

Цитирования и источники

Цитирований: 2Использованных источников: 0

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