Статья

Hierarchical Nanogaps within Bioscaffold Arrays as a High-Performance SERS Substrate for Animal Virus Biosensing

Feng ShaoState Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, ChinaZhicheng LüState Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, ChinaChen LiuState Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, ChinaHeyou HanState Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, ChinaKun ChenState Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, ChinaWentao LiState Key Laboratory of Agricultural Microbiology, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaQigai HeState Key Laboratory of Agricultural Microbiology, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaHui PengState Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, ChinaJuanni ChenState Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, China

2013en

ABI

Аннотация

A three-dimensional (3D) biomimetic SERS substrate with hierarchical nanogaps was formed on the bioscaffold arrays of cicada wings by one-step and reagents-free ion-sputtering techniques. This approach requires a minimal fabrication effort and cost and offers Ag nanoislands and Ag nanoflowers with four types of nanogaps (<10 nm) on the chitin nanopillars to generate a high density of hotspots (∼2000/μm2). The 3D biomimetic substrate shows a low detection limit to Rhodamine 6G (10(-13) M), high average enhancement factor (EF, 5.8×10(7)), excellent signal uniformity (5.4%), good stability, and suitability in biosensing. Furthermore, the finite-difference time-domain (FDTD) electric-field-distribution simulations illustrate that the 3D biomimetic SERS substrate provides the high-density hotspot area within a detection volumem, resulting in enormous SERS enhancement. In addition, the conspicuous far-field plasmon resonance peaks were not found to be a strong requirement for a high EF in 3D biomimetic substrates. Additionally, the novel substrate was applied in label-free animal viruses detection and differentiation with small amounts (1.0 μL) and low concentrations of analyte (1×10(3) PFU/mL), and it exhibited potential as an effective SERS platform for virus detection and sensing.

Перевод пока недоступен

Идентификаторы

DOI: 10.1021/am4045212

Цитирования и источники

Цитирований: 3Использованных источников: 0

Показатели — AkademScholar