Статья

A Multinuclear Metal Complex Based DNase‐Mimetic Artificial Enzyme: Matrix Cleavage for Combating Bacterial Biofilms

Zhaowei ChenJoint Department of Biomedical Engineering University of North Carolina at Chapel Hill and North Carolina State University Raleigh NC 27695 USAHaiwei JiLaboratory of Chemical Biology and State Key Laboratory of Rare Earth Resources Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun Jilin 130022 ChinaChaoqun LiuLaboratory of Chemical Biology and State Key Laboratory of Rare Earth Resources Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun Jilin 130022 ChinaWei BingUniversity of Chinese Academy of Sciences, Beijing, 100039 ChinaZhenzhen WangLaboratory of Chemical Biology and State Key Laboratory of Rare Earth Resources Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun Jilin 130022 ChinaXiaogang QuLaboratory of Chemical Biology and State Key Laboratory of Rare Earth Resources Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun Jilin 130022 China

2016en

ABI

Аннотация

Extracellular DNA (eDNA) is an essential structural component during biofilm formation, including initial bacterial adhesion, subsequent development, and final maturation. Herein, the construction of a DNase-mimetic artificial enzyme (DMAE) for anti-biofilm applications is described. By confining passivated gold nanoparticles with multiple cerium(IV) complexes on the surface of colloidal magnetic Fe3 O4 /SiO2 core/shell particles, a robust and recoverable artificial enzyme with DNase-like activity was obtained, which exhibited high cleavage ability towards both model substrates and eDNA. Compared to the high environmental sensitivity of natural DNase in anti-biofilm applications, DMAE exhibited a much better operational stability and easier recoverability. When DMAE was coated on substratum surfaces, biofilm formation was inhibited for prolonged periods of time, and the DMAE excelled in the dispersion of established biofilms of various ages. Finally, the presence of DMAE remarkably potentiated the efficiency of traditional antibiotics to kill biofilm-encased bacteria and eradiate biofilms.

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Идентификаторы

DOI: 10.1002/anie.201605296

Цитирования и источники

Цитирований: 2Использованных источников: 0

Показатели — AkademScholar