Статья

Comparison of Two Schizophyllum commune Strains in Production of Acetylcholinesterase Inhibitors and Antioxidants from Submerged Cultivation

Jovana MiškovićDepartment of Biology and Ecology, Faculty of Sciences, University of Novi Sad, TrgDositejaObradovića 2, 21000 Novi Sad, SerbiaMaja KaramanDepartment of Biology and Ecology, Faculty of Sciences, University of Novi Sad, TrgDositejaObradovića 2, 21000 Novi Sad, SerbiaMilena RašetaDepartment of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 3, 21000 Novi Sad, SerbiaNenad KrsmanovićDepartment of Biology and Ecology, Faculty of Sciences, University of Novi Sad, TrgDositejaObradovića 2, 21000 Novi Sad, SerbiaSanja BerežniDepartment of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 3, 21000 Novi Sad, SerbiaDragica JakovljevićInstitute of Chemistry, Technology and Metallurgy, University of Belgrade, Njegoševa 12, 11000 Belgrade, SerbiaFederica PiattoniLaboratory of Genetics & Genomics of Marine Resources and Environment (GenoDream), Department Biological, Geological & Environmental Sciences (BiGeA), University of Bologna, Via S. Alberto 163, 48123 Ravenna, ItalyAlessandra ZambonelliDipartimento di Scienze e Tecnologie Agroalimentari, University of Bologna, Via Fanin 46, 40127 Bologna, ItalyMaria Letizia GarganoDepartment of Agricultural and Environmental Science, University of Bari “Aldo Moro”, Via Amendola 165/A, I-70126 Bari, ItalyGiuseppe VenturellaDepartment of Agricultural, Food and Forest Sciences, University of Palermo, Via delle Scienze, Bldg. 4, 90128 Palermo, Italy

2021en

ABI

Аннотация

In recent years, fungi have been recognized as producers of acetylcholinesterase (AChE) inhibitors, agents important for the prevention of Alzheimer’s disease (AD). This study aimed to examine the AChE inhibitory, the antioxidative and antibacterial activity of two different Schizophyllum commune strains that originated from Serbia (SRB) and Italy (IT). Submerged cultivation of grown mycelia (M) and fermentation broth (F) of ethanol (EtOH) and polysaccharide (PSH) extracts lasted for 7, 14, 21 and 28 days. For AChE activity Ellman method was performed, while for antioxidative activity, sevendifferent assays were conducted: DPPH, ABTS, FRAP, SOA, OH, NO together with total phenolic content. Antimicrobial screen, LC–MS/MS technique and FTIR measurements were performed. Different isolates exhibited different AChE activity, with PSH being the strongest (SRB, M, 28 days IC90 79.73 ± 26.34 µg/mL), while in EtOH extracts, IT stood out (F, 14 days, IC50 0.8 ± 0.6 µg/mL). PSH extracts (7 days) exhibit significant antioxidative activity (AO), opposite to EtOH extracts where 14 and 21days periods stood out. Only tw extracts showed antibacterial activity. Following LC–MS/MS analysis p-hydroxybenzoic and gallic acids were the most abundant phenolics. PSH extracts demonstrated remarkable results, making this study debut and introducing S. commune as a valuable resource of AChE inhibitors.

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Идентификаторы

DOI: 10.3390/jof7020115

Цитирования и источники

Цитирований: 4Использованных источников: 0

Показатели — AkademScholar