Статья

Sensitive and selective electrochemical determination of uric acid in urine based on ultrasmall iron oxide nanoparticles decorated urchin-like nitrogen-doped carbon

Tingfan TangGuangxi Key Laboratory of Green Processing of Sugar Resources, College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou 545006, Guangxi, PR China; Institute for Ecological Research and Pollution Control of Plateau Lakes, School of Ecology and Environmental Science, Yunnan University, Kunming 650504, PR ChinaMenglin ZhouGuangxi Key Laboratory of Green Processing of Sugar Resources, College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou 545006, Guangxi, PR ChinaJiapei LvState Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, ChinaHao ChengGuangxi Key Laboratory of Green Processing of Sugar Resources, College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou 545006, Guangxi, PR China. Electronic address: [email protected]Huaisheng WangSchool of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252000, ChinaDanfeng QinGuangxi Key Laboratory of Green Processing of Sugar Resources, College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou 545006, Guangxi, PR ChinaGuangzhi HuInstitute for Ecological Research and Pollution Control of Plateau Lakes, School of Ecology and Environmental Science, Yunnan University, Kunming 650504, PR China; Department of Physics, Umeå University, Umeå 901 87, Sweden. Electronic address: [email protected]Xiaoyan LiuKey Laboratory of Anti-inflammatory and Immune Medicines, Ministry of Education, School of Pharmacy, Anhui Medical University, Hefei 230032, PR China. Electronic address: [email protected]

2022en

ABI

Аннотация

Hypercrosslinked pyrrole was synthesized via the Friedel–Crafts reaction and then carbonized to obtain urchin-like nitrogen-doped carbon (UNC). Ultrasmall iron oxide nanoparticles were then supported on UNC, and the composite was used to prepare an electrochemical sensor for detecting uric acid (UA) in human urine. FexOy/UNC was characterized and analyzed via scanning electron microscopy, transmission electron microscopy, energy dispersive spectrometry, X-ray diffraction, and X-ray photoelectron spectroscopy. A glassy carbon electrode (GCE) modified with FexOy/UNC was used as an electrochemical sensor to effectively identify UA. The electrochemical behavior of the FexOy/UNC-based UA sensor was studied using differential pulse stripping voltammetry, and the optimal conditions were determined by changing the amount of FexOy/UNC, pH of the buffer solution, deposition potential, and deposition time. Under optimal conditions, the FexOy/UNC-based electrochemical sensor detected UA in the range of 2–200 μM, where the limit of detection (LOD) for UA was 0.29 μM. Anti-interference experiments were performed, and the sensor was applied to the actual analysis of human urine samples. Urea, glucose, ascorbic acid, and many cations and anions present at 100-fold concentrations relative to UA did not strongly interfere with the response of the sensor to UA. The FexOy/UNC electrochemical sensor has high sensitivity and selectivity for uric acid in human urine samples and can be used for actual clinical testing of UA in urine.

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Идентификаторы

DOI: 10.1016/j.colsurfb.2022.112538

Цитирования и источники

Цитирований: 3Использованных источников: 0

Показатели — AkademScholar