Статья

The Influence of Cell Type and Culture Medium on Determining Cancer Selectivity of Cold Atmospheric Plasma Treatment

Eline BiscopPLASMANT Research Group, Department of Chemistry, University of Antwerp, 2610 Antwerp, BelgiumAbraham LinCenter for Oncological Research, University of Antwerp, 2610 Antwerp, BelgiumWilma Van BoxemPLASMANT Research Group, Department of Chemistry, University of Antwerp, 2610 Antwerp, BelgiumJinthe Van LoenhoutCenter for Oncological Research, University of Antwerp, 2610 Antwerp, BelgiumJoey De BackerDepartment of Biomedical Sciences, University of Antwerp, 2610 Antwerp, BelgiumChristophe DebenCenter for Oncological Research, University of Antwerp, 2610 Antwerp, BelgiumSylvia DewildeDepartment of Biomedical Sciences, University of Antwerp, 2610 Antwerp, BelgiumEvelien SmitsCenter for Oncological Research, University of Antwerp, 2610 Antwerp, BelgiumAnnemie BogaertsPLASMANT Research Group, Department of Chemistry, University of Antwerp, 2610 Antwerp, Belgium

2019en

ABI

Аннотация

Increasing the selectivity of cancer treatments is attractive, as it has the potential to reduce side-effects of therapy. Cold atmospheric plasma (CAP) is a novel cancer treatment that disrupts the intracellular oxidative balance. Several reports claim CAP treatment to be selective, but retrospective analysis of these studies revealed discrepancies in several biological factors and culturing methods. Before CAP can be conclusively stated as a selective cancer treatment, the importance of these factors must be investigated. In this study, we evaluated the influence of the cell type, cancer type, and cell culture medium on direct and indirect CAP treatment. Comparison of cancerous cells with their non-cancerous counterparts was performed under standardized conditions to determine selectivity of treatment. Analysis of seven human cell lines (cancerous: A549, U87, A375, and Malme-3M; non-cancerous: BEAS-2B, HA, and HEMa) and five different cell culture media (DMEM, RPMI1640, AM, BEGM, and DCBM) revealed that the tested parameters strongly influence indirect CAP treatment, while direct treatment was less affected. Taken together, the results of our study demonstrate that cell type, cancer type, and culturing medium must be taken into account before selectivity of CAP treatment can be claimed and overlooking these parameters can easily result in inaccurate conclusions of selectivity.

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Идентификаторы

DOI: 10.3390/cancers11091287

Цитирования и источники

Цитирований: 3Использованных источников: 0

Показатели — AkademScholar