Статья

Fabrication of magnetic niosomal platform for delivery of resveratrol: potential anticancer activity against human pancreatic cancer Capan-1 cell

Akram Firouzi AmandiDepartment of Medical Immunology, Facultyof Medicine, Tabriz University of Medical Sciences, Tabriz, IranZahra BahmanyarSchool of Pharmacy, Shiraz University of Medical Sciences, Shiraz, IranMehdi DadashpourCancer Research Center, Semnan University of Medical Sciences, Semnan, Iran. [email protected]Mehrnoosh LakSchool of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranMohammad NatamiDepartment of Urology, Shahid Mohammadi Hospital, Hormozgan University of Medical Sciences, Bandar Abbas, IranYusuf DöğüşDepartment of Medical Biochemistry, Faculty of Medicine, Cukurova University, Adana, TurkeyMahsa AlemDepartment of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, IranOmid Ali AdeliDepartment of Pathology, Lorestan University of Medical Sciences, Khorramabad, Iran. [email protected]

2024en

ABI

Аннотация

Recently, the presence of different nanoparticles (NPs) has developed targeting drug delivery in treatment of cancer cell. Targeted drug delivery systems using NPs have shown great promise in improving the efficacy of intracellular uptake as well as local concentration of therapeutics with minimizing side effects. The current study planned to synthesized resveratrol-loaded magnetic niosomes nanoparticles (RSV-MNIONPs) and evaluate their cytotoxicity activity in pancreatic cancer cells. For this aim, magnetic nanoparticles (MNPs) were synthesized and loaded into niosomes (NIOs) by the thin film hydration technique and then characterized via DLS, FT-IR, TEM, SEM and VSM techniques. Moreover, the cytotoxic activity of the RSV-MNIONPs on the Capan-1 cells line was assessed by the MTT test. The distribution number of RSV-MNIONPs was gained about 80 nm and 95 nm with surface charge of - 14.0 mV by SEM and TEM analysis, respectively. RSV loading efficacy in NIOs was about 85%, and the drug releases pattern displayed a sustained discharge with a maximum amount about 35% and 40%, within 4 h in pH = 7.4 and pH = 5.8, respectively. The cytotoxicity of the RSV-MNIONPs in the presence of an external magnetic field is higher than that of the RSV, indicating enhanced cellular uptake in their encapsulated states. Furthermore, RSV loaded MNNPs were found to induce more cell cycle arrest at the G0/G1 checkpoint than free RSV. Compared with RSV-treated cells, the mRNA expression levels of BAX, Bcl2, FAS, P 53, Cyclin D and hTERT, were significantly changed in cells treated with RSV loaded MNNPs. The niosomes NPs approaches have been widely used to attain higher solubility, improved bioavailability, enhanced stability, and control delivery of RSV. Our formulation displayed antitumor activity and can be considered an appropriate carrier with a great potential for future usage in cancer therapy.

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Идентификаторы

DOI: 10.1186/s12935-024-03219-2

Цитирования и источники

Цитирований: 3Использованных источников: 0

Показатели — AkademScholar