Статья

Green Synthesis of Zinc Oxide Nanoparticles from Althaea officinalis Flower Extract Coated with Chitosan for Potential Healing Effects on Diabetic Wounds by Inhibiting TNF-α and IL-6/IL-1β Signaling Pathways

Sammar Fathy ElhabalDepartment of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Modern University for Technology and Information (MTI), Mokattam, Cairo, EgyptNashwa AbdelaalDepartment of Integrative Physiology, Baylor College of Medicine, Houston, TX, USASaeed A. S. Al-ZuhairyDepartment of Pharmacy, Kut University College, Kut, Wasit, IraqMohamed Fathi Mohamed ElrefaiDepartment of Anatomy and Embryology, Faculty of Medicine, Ain Shams University, Cairo, EgyptAhmed M. HamdanDepartment of Pharmacy Practice, Faculty of Pharmacy, University of Tabuk, Tabuk, Saudi ArabiaMohamed Mansour KhalifaDepartment of Human Physiology, Faculty of Medicine, Cairo University, Cairo, EgyptSandra HababehDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaMohammad Ahmad KhasawnehGehad KhamisDepartment of Clinical Pharmacology, Faculty of Medicine, Alexandria University, Alexandria, EgyptJakline NelsonDepartment of Microbiology and Immunology, Faculty of Pharmacy, Nahda University, Beni-Suef, EgyptPassant MohieDepartment of Clinical Pharmacology, Faculty of Medicine, Alexandria University, Alexandria, EgyptRania A. GadDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Nahda University, Beni-Suef, EgyptAmira RizkFood Science and Technology Department, Faculty of Agricultural, Tanta University, Tanta, EgyptSoad L. KabilDepartment of Clinical Pharmacology, Faculty of Medicine, Zagazig University, Zagazig, EgyptMohamed El-AsheryMedicinal Chemistry Department, Faculty of Pharmacy, King Salman International University, Ras-Sedr, South Sinai, EgyptBhaskara JastiDepartment of Pharmaceutics and Medicinal Chemistry, Thomas J. Long School of Pharmacy, University of the Pacific, Stockton, CA, USANahla A. ElzohairyAir Force Specialized Hospital, Cairo, EgyptTayseer ElnawawyDepartment of Pharmaceutics, Egyptian Drug Authority, Cairo, EgyptFatma E. HassanGeneral Medicine Practice Program, Department of Physiology, Batterjee Medical College, Jeddah, Saudi ArabiaMohamed El- NabarawiDepartment of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Cairo, Egypt

2024en

ABI

Аннотация

Background: Diabetes Mellitus is a multisystem chronic pandemic, wound inflammation, and healing are still major issues for diabetic patients who may suffer from ulcers, gangrene, and other wounds from uncontrolled chronic hyperglycemia. Marshmallows or Althaea officinalis (A.O.) contain bioactive compounds such as flavonoids and phenolics that support wound healing via antioxidant, anti-inflammatory, and antibacterial properties. Our study aimed to develop a combination of eco-friendly formulations of green synthesis of ZnO-NPs by Althaea officinalis extract and further incorporate them into 2% chitosan (CS) gel. Method and Results: First, develop eco-friendly green Zinc Oxide Nanoparticles (ZnO-NPs) and incorporate them into a 2% chitosan (CS) gel. In-vitro study performed by UV-visible spectrum analysis showed a sharp peak at 390 nm, and Energy-dispersive X-ray (EDX) spectrometry showed a peak of zinc and oxygen. Besides, Fourier transforms infrared (FTIR) was used to qualitatively validate biosynthesized ZnO-NPs, and transmission electron microscope (TEM) showed spherical nanoparticles with mean sizes of 76 nm and Zeta potential +30mV. The antibacterial potential of A.O.-ZnO-NPs-Cs was examined by the diffusion agar method against Gram-positive ( Staphylococcus aureus and Bacillus subtilis ) and Gram-negative bacteria ( Escherichia coli and Pseudomonas aeruginosa ). Based on the zone of inhibition and minimal inhibitory indices (MIC). In addition, an in-silico study investigated the binding affinity of A.O . major components to the expected biological targets that may aid wound healing. Althaea Officinalis , A.O-ZnO-NPs group showed reduced downregulation of IL-6, IL-1β, and TNF-α and increased IL-10 levels compared to the control group signaling pathway expression levels confirming the improved anti-inflammatory effect of the self-assembly method. In-vivo study and histopathological analysis revealed the superiority of the nanoparticles in reducing signs of inflammation and wound incision in rat models. Conclusion: These biocompatible green zinc oxide nanoparticles, by using Althaea Officinalis chitosan gel ensure an excellent new therapeutic approach for quickening diabetic wound healing. Keywords: wound healing, antimicrobial, antioxidant, Staphylococcus aureus , Bacillus subtilis , Escherichia coli , Pseudomonas aeruginosa , chitosan, wound concentration, wound incision

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Идентификаторы

DOI: 10.2147/ijn.s455270

Цитирования и источники

Цитирований: 2Использованных источников: 0

Показатели — AkademScholar