Статья

A New Water-Soluble Nanomicelle Formed through Self-Assembly of Pectin–Curcumin Conjugates: Preparation, Characterization, and Anticancer Activity Evaluation

Feng BaiChemistry and Chemical Engineering, Nanjing University of Technology, Nanjing City, Jiangsu Province 210009, People’s Republic of ChinaJiajing DiaoInstitute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People’s Republic of ChinaYing WangChemistry and Chemical Engineering, Nanjing University of Technology, Nanjing City, Jiangsu Province 210009, People’s Republic of ChinaShixin SunInstitute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People’s Republic of ChinaHongmei ZhangInstitute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People’s Republic of ChinaYunyun LiuInstitute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People’s Republic of ChinaYanqing WangChemistry and Chemical Engineering, Nanjing University of Technology, Nanjing City, Jiangsu Province 210009, People’s Republic of ChinaJian CaoInstitute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People’s Republic of China

2017en

ABI

Аннотация

Curcumin is a dominating active component of Curcuma longa and has been studied widely because of its prominent biological activities. The extremely low aqueous solubility, stability, and bioavailability of curcumin limit its application in the field of medicine. In this study, we developed pectin–curcumin (PEC–CCM) conjugates that could self-assemble water-soluble nanomicelles in aqueous solution. The structure of PEC–CCM conjugates was characterized by ultraviolet–visible spectra, fluorescence spectra, Fourier transform infrared spectroscopy, and 1H nuclear magnetic resonance spectroscopy. The thermal property of PEC–CCM conjugates was investigated by thermogravimetric analysis. It was found that PEC–CCM conjugates had formed nanomicelles in aqueous medium via self-assembly. These nanomicelles were observed as small spheres or ellipsoids and aggregated with a size range of 70–190 nm by transmission electron microscopy analysis. In a solution of nanomicelles, the stability of curcumin was improved, and its antioxidant property was preserved. The anticancer activity of PEC–CCM conjugates was quantified by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay using a hepatic cancer cell line (HepG2), a breast cancer cell line (MCF-7), a cervical cancer cell line (HeLa), and a human normal kidney cell line (293A). It was found that the curcumin of PEC–CCM conjugates had a more significant inhibitory effect on cancer cells and was less cytotoxic to normal cells than free curcumin was. PEC–CCM conjugates have great potential for some food and pharmaceutical applications.

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Идентификаторы

DOI: 10.1021/acs.jafc.7b02250

Цитирования и источники

Цитирований: 2Использованных источников: 0

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