Статья

Hemoglobin Adducts and Mercapturic Acid Excretion of Acrylamide and Glycidamide in One Study Population

Eva C. HartmannInstitute and Outpatient Clinic of Occupational, Social and Environmental Medicine, University of Erlangen−Nuremberg, Schillerstrasse 25/29, D-91054 Erlangen, Germany; Institute and Outpatient Clinic of Occupational and Social Medicine, University Hospital, Aachen University of Technology, Pauwelsstrasse 30, D-52074 Aachen, Germany; and Department of Environmental Health, Bavarian Health and Safety Authority, Veterinaerstrasse 2, D-85764 Oberschleissheim, GermanyMelanie I. BoettcherInstitute and Outpatient Clinic of Occupational, Social and Environmental Medicine, University of Erlangen−Nuremberg, Schillerstrasse 25/29, D-91054 Erlangen, Germany; Institute and Outpatient Clinic of Occupational and Social Medicine, University Hospital, Aachen University of Technology, Pauwelsstrasse 30, D-52074 Aachen, Germany; and Department of Environmental Health, Bavarian Health and Safety Authority, Veterinaerstrasse 2, D-85764 Oberschleissheim, GermanyThomas SchettgenInstitute and Outpatient Clinic of Occupational, Social and Environmental Medicine, University of Erlangen−Nuremberg, Schillerstrasse 25/29, D-91054 Erlangen, Germany; Institute and Outpatient Clinic of Occupational and Social Medicine, University Hospital, Aachen University of Technology, Pauwelsstrasse 30, D-52074 Aachen, Germany; and Department of Environmental Health, Bavarian Health and Safety Authority, Veterinaerstrasse 2, D-85764 Oberschleissheim, GermanyHermann FrommeInstitute and Outpatient Clinic of Occupational, Social and Environmental Medicine, University of Erlangen−Nuremberg, Schillerstrasse 25/29, D-91054 Erlangen, Germany; Institute and Outpatient Clinic of Occupational and Social Medicine, University Hospital, Aachen University of Technology, Pauwelsstrasse 30, D-52074 Aachen, Germany; and Department of Environmental Health, Bavarian Health and Safety Authority, Veterinaerstrasse 2, D-85764 Oberschleissheim, GermanyHans DrexlerInstitute and Outpatient Clinic of Occupational, Social and Environmental Medicine, University of Erlangen−Nuremberg, Schillerstrasse 25/29, D-91054 Erlangen, Germany; Institute and Outpatient Clinic of Occupational and Social Medicine, University Hospital, Aachen University of Technology, Pauwelsstrasse 30, D-52074 Aachen, Germany; and Department of Environmental Health, Bavarian Health and Safety Authority, Veterinaerstrasse 2, D-85764 Oberschleissheim, GermanyJ. AngererInstitute and Outpatient Clinic of Occupational, Social and Environmental Medicine, University of Erlangen−Nuremberg, Schillerstrasse 25/29, D-91054 Erlangen, Germany; Institute and Outpatient Clinic of Occupational and Social Medicine, University Hospital, Aachen University of Technology, Pauwelsstrasse 30, D-52074 Aachen, Germany; and Department of Environmental Health, Bavarian Health and Safety Authority, Veterinaerstrasse 2, D-85764 Oberschleissheim, Germany

2008en

ABI

Аннотация

The aim of this study was to determine the relationship between the oxidative and reductive metabolic pathways of acrylamide (AA) in the nonsmoking general population. For the first time both the blood protein adducts and the urinary metabolites of AA and glycidamide (GA) were quantified in an especially designed study group with even distribution of age and gender. The hemoglobin adducts N-carbamoylethylvaline (AAVal) and N-( R, S)-2-hydroxy-2-carbamoylethylvaline (GAVal) were detected by GC-MS/MS in all blood samples with median levels of 30 and 34 pmol/g of globin, respectively. Concentrations ranged from 15 to 71 pmol/g of globin for AAVal and from 14 to 66 pmol/g of globin for GAVal. The ratio GAVal/AAVal was 0.4-2.7 (median = 1.1). The urinary metabolites were determined by LC-MS/MS. Of all urine samples examined 99% of N-acetyl- S-(2-carbamoylethyl)- l-cysteine (AAMA) levels and 73% of N-( R/ S)-acetyl- S-(2-carbamoyl-2-hydroxyethyl)- l-cysteine (GAMA) levels were above the LOD (1.5 microg/L). Concentrations ranged from <LOD to 229 microg/L (median = 29 microg/L) for AAMA and from <LOD to 85 microg/L (median = 7 microg/L) for GAMA. The ratio of GAMA/AAMA varied from 0.004 to 1.4 (median = 0.3). Using hemoglobin adduct levels in blood and mercapturic acid excretion in urine for calculation of daily AA intake gave practically identical values. The median daily intakes were 0.43 (0.21-1.04) microg/kg of body weight(bw)/day using Hb adducts and 0.51 (<LOD-2.32) microg/kg of bw/day using mercapturic acids for calculations. Children take up approximately 1.3-1.5 times more AA per kilogram of body weight than adults. The ratio GAMA/AAMA is significantly higher in the group of young children (6-10 years) with a median level of 0.5. A gender-related difference in internal exposure and metabolism was not observed.

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Идентификаторы

DOI: 10.1021/jf800277h

Цитирования и источники

Цитирований: 2Использованных источников: 0

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