Статья

Mangiferin (mango) attenuates AOM-induced colorectal cancer in rat’s colon by augmentation of apoptotic proteins and antioxidant mechanisms

Khaled Abdul‐Aziz AhmedDepartment of Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Al-Ahliyya Amman University, Amman, JordanAhmed Aj. JabbarDepartment of Medical Laboratory Technology, Erbil Technical Health and Medical College, Erbil Polytechnic University, Erbil, 44001, Iraq. [email protected]Mahmood Ameen AbdullaDepartment of Medical Microbiology, College of Science, Cihan University-Erbil, Erbil, Kurdistan Region, IraqZaenah Zuhair AlamriDepartment of Biological Science, College of Science, University of Jeddah, P.O. Box 80327, Jeddah, 21589, Saudi ArabiaNur Ain SalehenDepartment of Biomedical Sciences, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, MalaysiaIbrahim Abdel Aziz IbrahimDepartment of Pharmacology and Toxicology, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi ArabiaGhassan AlmaimaniDepartment of Surgery, Faculty of Medicine, Umm Al-Qura University, Al Abdeyah, PO Box 7607, Makkah, Saudi ArabiaGhazi A. BamagousDepartment of Pharmacology and Toxicology, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi ArabiaRiyad A. AlmaimaniDepartment of Biochemistry, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi ArabiaHussain A. AlmasmoumDepartment of Clinical Laboratory Science, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah, Saudi ArabiaMazen M. GhaithDepartment of Clinical Laboratory Science, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah, Saudi ArabiaWesam F. FarrashDepartment of Clinical Laboratory Science, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah, Saudi Arabia

2024en

ABI

Аннотация

Mangiferin (MF) is a natural C-glucosylxantone compound that has many substantial curative potentials against numerous illnesses including cancers. The present study's goal is to appraise the chemo preventive possessions of MF on azoxymethane (AOM)-mediated colonic aberrant crypt foci (ACF) in rats. Rats clustered into 5 groups, negative control (A), inoculated subcutaneously with normal saline twice and nourished on 0.5% CMC; groups B-E injected twice with 15 mg/kg azoxymethane followed by ingestion of 0.5% CMC (B, cancer control); intraperitoneal inoculation of 35 mg/kg 5-fluorouracil (C, reference rats) or nourished on 30 mg/kg (D) and 60 mg/kg (E) of MF. Results of gross morphology of colorectal specimens showed significantly lower total colonic ACF incidence in MF-treated rats than that of cancer controls. The colon tissue examination of cancer control rats showed increased ACF availability with bizarrely elongated nuclei, stratified cells, and higher depletion of the submucosal glands compared to MF-treated rats. Mangiferin treatment caused increased regulation of pro-apoptotic (increased Bax) proteins and reduced the β-catenin) proteins expression. Moreover, rats fed on MF had significantly higher glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), and lower malondialdehyde (MDA) concentrations in their colonic tissue homogenates. Mangiferin supplementation significantly down-shifted pro-inflammatory cytokines (transforming growth factor-α and interleukine-6) and up-shifted anti-inflammatory cytokines (interleukine-10) based on serum analysis. The chemo-protective mechanistic of MF against AOM-induced ACF, shown by lower ACF values and colon tissue penetration, could be correlated with its positive modulation of apoptotic cascade, antioxidant enzymes, and inflammatory cytokines originating from AOM oxidative stress insults.

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Идентификаторы

DOI: 10.1038/s41598-023-50947-y

Цитирования и источники

Цитирований: 2Использованных источников: 0

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