Effect of Polyphenols Isolated from Plantago major L. and Plantago lanceolata L. on Mitochondrial Permeability Transition Pore in Rat Liver

It has been shown that the transport of electrons in the respiratory chain of mitochondria, ion-transport processes, the balance of Ca2+ ions and other metabolic processes in the cell are intrinsically connected with lipoperoxidation processes. In particular, in recent years, plant biologically active compounds have been widely used to eliminate oxidative processes developing in the body. Accordingly, we considered it important to study the mechanisms of action of xylopyr-T, glucopyr-T and 2-DVG polyphenols isolated from the plants Plantago major L. and Plantago lanceolata L., belonging to the Plantaginaceae family, which are widely used in folk medicine. Therefore, in vitro experiments, the amount of malondialdehyde and the condition state of mPTP were studied by spectrophotometric method. The obtained results revealed that the studied polyphenols inhibit lipoperoxidation induced by Fe2+/ascorbate the production of malondialdehyde was reduce by xylopyr-T to 81.9 ± 1.2 %, by glucopyr-T to 79.4 ± 1.7 % and by 2-DVG to 75.0 ± 1.8 % at a concentration of 4 µM in rat liver mitochondria. At the same time, mPTP opening in the medium with the substrate succinate (+ rotenone) inhibition 2-DVG polyphenol at a concentration of 100 μM was reduced by 84.5 ± 2.8 %, xylopyr-T polyphenol at a concentration of 200 μM by 67.6 ± 4.8 % and it was shown that glucopyr-T polyphenol at a concentration of 200 µM by 32.5 ± 1.65 % compared to the control. In conclusion, it can be illustrated that the studied polyphenols, along with their high antioxidant properties, show a structure-dependent inhibition of mPTP opening, which is involved in various physiological and pathological processes. HIGHLIGHTS In this study, hydrolysable tannins, which have strong antioxidant properties isolated from Plantago plants, were investigated and confirmed by MDA analysis in vitro These hydrolyzable tannins exhibited substrate-dependent inhibition of rat liver mitochondrial PTP in vitro It can be assumed from the data obtained that these hydrolyzable tannins act organ-specifically GRAPHICAL ABSTRACT

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