Статья

A Disposable Saliva Electrochemical MIP-Based Biosensor for Detection of the Stress Biomarker α-Amylase in Point-of-Care Applications

Tânia S.C.R. RebeloCIQUP—Chemistry Research Center, Department of Chemistry and Biochemistry Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, s/n, 4169-007 Porto, PortugalInês M. MirandaCIQUP—Chemistry Research Center, Department of Chemistry and Biochemistry Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, s/n, 4169-007 Porto, PortugalAna T. S. C. BrandãoCIQUP—Chemistry Research Center, Department of Chemistry and Biochemistry Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, s/n, 4169-007 Porto, PortugalLaura G. SousaCIQUP—Chemistry Research Center, Department of Chemistry and Biochemistry Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, s/n, 4169-007 Porto, PortugalJosé A. RibeiroCIQUP—Chemistry Research Center, Department of Chemistry and Biochemistry Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, s/n, 4169-007 Porto, PortugalFernando SilvaCIQUP—Chemistry Research Center, Department of Chemistry and Biochemistry Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, s/n, 4169-007 Porto, PortugalCarlos M. PereiraCIQUP—Chemistry Research Center, Department of Chemistry and Biochemistry Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, s/n, 4169-007 Porto, Portugal

2021en

ABI

Аннотация

The design and synthesis of artificial receptors based on molecular imprinting (MI) technology for the development of a new MIP-based biosensor for detection of the stress biomarker α-amylase in human saliva in point-of-care (PoC) applications is described in this work. The portable electrochemical devices for monitoring α-amylase consists of cost-effective and disposable gold screen-printed electrodes (AuSPEs). To build the electrochemical device, the template biomolecule was firstly immobilized directly over the working area of the gold chip previously activated with a self-assembled monolayer (SAM) of cysteamine (CA). Then, pyrrole (Py) monomer was selected as building block of a polymeric network prepared by CV electropolymerization. After the electropolymerization process, the enzyme was removed from the polymer film in order to build the specific recognition sites for the target enzyme. The MIP biosensor showed a very wide linear concentration range (between 3.0 × 10−4 to 0.60 mg mL−1 in buffer solution and between 3.0 × 10−4 to 3.0 × 10−2 mg mL−1 in human saliva) and low detection levels were achieved (LOD < 3.0 × 10−4 mg mL−1) using square wave voltammetry (SWV) as the electroanalytical technique.

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Идентификаторы

DOI: 10.3390/electrochem2030028

Цитирования и источники

Цитирований: 2Использованных источников: 0

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