Статья

Hordenine: A Novel Quorum Sensing Inhibitor and Antibiofilm Agent against <i>Pseudomonas aeruginosa</i>

Jinwei ZhouSchool of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, ChinaHuaizhi LuoSchool of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, ChinaHuan JiangSchool of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, ChinaTing-Kun JianSchool of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, ChinaZiqian ChenSchool of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, ChinaAi‐Qun JiaState Key Laboratory of Marine Resource Utilization in South China Sea, Key Laboratory of Tropical Biological Resources of Ministry Education, Hainan University, Haikou 570228, China

2018en

ABI

Аннотация

The quorum sensing (QS) inhibitory activity of hordenine from sprouting barley against foodborne pathogen Pseudomonas aeruginosa was evaluated for the first time here. At concentrations ranging from 0.5 to 1.0 mg mL–1, hordenine inhibited the levels of acyl-homoserine lactones. The enhanced susceptibility of hordenine with netilmicin on P. aeruginosa PAO1 biofilm formation as well as their efficiency in disrupting preformed biofilms was also evaluated using scanning electron microscopy and confocal laser scanning microscopy (CLSM). Hordenine treatment inhibited the production of QS-related extracellular virulence factors of P. aeruginosa PAO1. Additionally, quantitative real-time polymerase chain reaction analysis demonstrated that the expressions of QS-related genes, lasI, lasR, rhlI, and rhlR, were significantly suppressed. Our results indicated that hordenine can serve as a competitive inhibitor for signaling molecules and act as a novel QS-based agent to defend against foodborne pathogens.

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Идентификаторы

DOI: 10.1021/acs.jafc.7b05035

Цитирования и источники

Цитирований: 2Использованных источников: 0

Показатели — AkademScholar