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Electroporation-induced siRNA precipitation obscures the efficiency of siRNA loading into extracellular vesicles

Sander A. A. KooijmansDepartment of Clinical Chemistry and Haematology, University Medical Center Utrecht, Utrecht, The NetherlandsStephan StremerschLaboratory of General Biochemistry and Physical Pharmacy, Ghent University, Ghent, BelgiumKevin BraeckmansLaboratory of General Biochemistry and Physical Pharmacy, Ghent University, Ghent, Belgium; Centre for Nano- and Biophotonics, Ghent University, Ghent, BelgiumStefaan C. De SmedtLaboratory of General Biochemistry and Physical Pharmacy, Ghent University, Ghent, BelgiumAn HendrixDepartment of Radiation Oncology and Experimental Cancer Research, Laboratory of Experimental Cancer Research, Ghent University Hospital, Ghent, BelgiumMatthew J. A. WoodDepartment of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UKRaymond M. SchiffelersDepartment of Clinical Chemistry and Haematology, University Medical Center Utrecht, Utrecht, The NetherlandsKoen RaemdonckLaboratory of General Biochemistry and Physical Pharmacy, Ghent University, Ghent, Belgium. Electronic address: [email protected]Pieter VaderDepartment of Clinical Chemistry and Haematology, University Medical Center Utrecht, Utrecht, The Netherlands; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK. Electronic address: [email protected]
2013en
ABI

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