Статья

Connexins regulate calcium signaling by controlling ATP release

Maria Luisa CotrinaDepartments of Cell Biology and Anatomy, Pathology, and Neurosurgery, New York Medical College, Valhalla, NY 10595; and Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario N6A 5C1, CanadaJane H.-C. LinDepartments of Cell Biology and Anatomy, Pathology, and Neurosurgery, New York Medical College, Valhalla, NY 10595; and Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario N6A 5C1, CanadaAlexandra Alves‐RodriguesDepartments of Cell Biology and Anatomy, Pathology, and Neurosurgery, New York Medical College, Valhalla, NY 10595; and Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario N6A 5C1, CanadaShujun LiuDepartments of Cell Biology and Anatomy, Pathology, and Neurosurgery, New York Medical College, Valhalla, NY 10595; and Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario N6A 5C1, CanadaJiang LiDepartments of Cell Biology and Anatomy, Pathology, and Neurosurgery, New York Medical College, Valhalla, NY 10595; and Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario N6A 5C1, CanadaHooman Azmi-GhadimiDepartments of Cell Biology and Anatomy, Pathology, and Neurosurgery, New York Medical College, Valhalla, NY 10595; and Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario N6A 5C1, CanadaJian KangDepartments of Cell Biology and Anatomy, Pathology, and Neurosurgery, New York Medical College, Valhalla, NY 10595; and Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario N6A 5C1, CanadaChristian C. NausDepartments of Cell Biology and Anatomy, Pathology, and Neurosurgery, New York Medical College, Valhalla, NY 10595; and Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario N6A 5C1, CanadaMaiken NedergaardDepartments of Cell Biology and Anatomy, Pathology, and Neurosurgery, New York Medical College, Valhalla, NY 10595; and Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario N6A 5C1, Canada

1998en

ABI

Аннотация

Forced expression of gap junction proteins, connexins, enables gap junction-deficient cell lines to propagate intercellular calcium waves. Here, we show that ATP secretion from the poorly coupled cell lines, C6 glioma, HeLa, and U373 glioblastoma, is potentiated 5- to 15-fold by connexin expression. ATP release required purinergic receptor-activated intracellular Ca2+ mobilization and was inhibited by Cl- channel blockers. Calcium wave propagation also was reduced by purinergic receptor antagonists and by Cl- channel blockers but insensitive to gap junction inhibitors. These observations suggest that cell-to-cell signaling associated with connexin expression results from enhanced ATP release and not, as previously believed, from an increase in intercellular coupling.

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Идентификаторы

DOI: 10.1073/pnas.95.26.15735

Цитирования и источники

Цитирований: 3Использованных источников: 0

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