Is the mammalian porin channel, VDAC, a perfect cylinder in the high conductance state?
Carlos M.M. CarneiroCenter of Biological Sciences, Department of Biophysics and Radiobiology, Federal University of Pernambuco, Recife, PE, BrazilOleg V. KrasilnikovLaboratory of Membrane Biophysics, Center of Biological Sciences, Department of Biophysics and Radiobiology, Federal University of Pernambuco, Av. prof. Moraes Rego, s/n, Cidade Universitaria, 50670-901 Recife, PE, BrazilLiliya N. YuldashevaLaboratory of Membrane Biophysics, Center of Biological Sciences, Department of Biophysics and Radiobiology, Federal University of Pernambuco, Av. prof. Moraes Rego, s/n, Cidade Universitaria, 50670-901 Recife, PE, BrazilAntônio Carlos Campos de CarvalhoInstitute of Biophysics Carlos Chagas Filho, UFRJ, Rio de Janeiro, RJ, BrazilR.A. NogueiraLaboratory of Membrane Biophysics, Center of Biological Sciences, Department of Biophysics and Radiobiology, Federal University of Pernambuco, Av. Prof. Moraes Rego, S/N, Cidade Universitaria, 50670-901 Recife, PE, Brazil
1997en
ABI
Аннотация
The mammalian porin channel (VDAC, porin-31BM) was reconstituted in planar lipid bilayers under voltage clamp conditions. The radii of both entrances of the channel were examined using a method that consisted in filling the channel with different non-electrolytes through its cis or trans entrances while recording single channel conductances. As a result it was found that the geometry of channels formed by porin-31BM could not be approximated by a perfectly cylindrical pore. In fact there is an asymmetry in the geometry of the channel: the diameters of the cis and trans entrances were estimated to be approximately 2 nm and approximately 4 nm respectively.
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