Статья

Fluorescent Nanodiamonds Embedded in Biocompatible Translucent Shells

Ivan ŘehořInstitute of Organic Chemistry and Biochemistry AS CR v.v.i. Flemingovo nam. 2 Prague 6 166 10 Czech RepublicJitka ŠlegerováInstitute of Organic Chemistry and Biochemistry AS CR v.v.i. Flemingovo nam. 2 Prague 6 166 10 Czech RepublicJan KučkaInstitute of Macromolecular Chemistry AS CR v.v.i. Heyrovského nám. 2 Prague 6 162 06 Czech RepublicVladimír ProksInstitute of Macromolecular Chemistry AS CR v.v.i. Heyrovského nám. 2 Prague 6 162 06 Czech RepublicVladimíra PetrákováFaculty of Biomedical Engineering Czech Technical University in Prague, Sítná sq. 3105, 272 01 Kladno (Czech Republic) and Institute of Physics AS CR v.v.i Prague 8 Czech RepublicMarie‐Pierre AdamLaboratoire de Photonique Quantique et Moléculaire UMR 8537 CNRS and ENS Cachan F‐94235 Cachan (France), Laboratoire Aimé Cotton, CNRS, Université Paris Sud and ENS Cachan F‐91405 Orsay FranceFrançois TreussartLaboratoire de Photonique Quantique et Moléculaire UMR 8537 CNRS and ENS Cachan F‐94235 Cachan (France), Laboratoire Aimé Cotton, CNRS, Université Paris Sud and ENS Cachan F‐91405 Orsay FranceStuart TurnerEMAT, University of Antwerp Groenenborgerlaan 171 B‐2020 Antwerp BelgiumSara BalsEMAT, University of Antwerp Groenenborgerlaan 171 B‐2020 Antwerp BelgiumPavel ŠáchaInstitute of Organic Chemistry and Biochemistry AS CR v.v.i. Flemingovo nam. 2 Prague 6 166 10 Czech RepublicM LedvinaInstitute of Organic Chemistry and Biochemistry AS CR v.v.i. Flemingovo nam. 2 Prague 6 166 10 Czech RepublicAmy M. WenDepartment of Biomedical Engineering Case Western Reserve University, School of Medicine and Engineering 10990 Euclid Avenue Cleveland Ohio USANicole F. SteinmetzDepartment of Biomedical Engineering Case Western Reserve University, School of Medicine and Engineering 10990 Euclid Avenue Cleveland Ohio USAPetr CíglerInstitute of Organic Chemistry and Biochemistry AS CR v.v.i. Flemingovo nam. 2 Prague 6 166 10 Czech Republic

2014en

ABI

Аннотация

High pressure high temperature (HPHT) nanodiamonds (NDs) represent extremely promising materials for construction of fluorescent nanoprobes and nanosensors. However, some properties of bare NDs limit their direct use in these applications: they precipitate in biological solutions, only a limited set of bio-orthogonal conjugation techniques is available and the accessible material is greatly polydisperse in shape. In this work, we encapsulate bright 30-nm fluorescent nanodiamonds (FNDs) in 10-20-nm thick translucent (i.e., not altering FND fluorescence) silica shells, yielding monodisperse near-spherical particles of mean diameter 66 nm. High yield modification of the shells with PEG chains stabilizes the particles in ionic solutions, making them applicable in biological environments. We further modify the opposite ends of PEG chains with fluorescent dyes or vectoring peptide using click chemistry. High conversion of this bio-orthogonal coupling yielded circa 2000 dye or peptide molecules on a single FND. We demonstrate the superior properties of these particles by in vitro interaction with human prostate cancer cells: while bare nanodiamonds strongly aggregate in the buffer and adsorb onto the cell membrane, the shell encapsulated NDs do not adsorb nonspecifically and they penetrate inside the cells.

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Идентификаторы

DOI: 10.1002/smll.201302336

Цитирования и источники

Цитирований: 2Использованных источников: 0

Показатели — AkademScholar