Статья

Many Parallel Losses of <i>infA</i> from Chloroplast DNA during Angiosperm Evolution with Multiple Independent Transfers to the Nucleus

Ronny S. MillenDepartment of Environmental, Population, and Organismic Biology, University of Colorado, Boulder, Colorado 80309Richard G. OlmsteadDepartment of Botany, University of Washington, Seattle, Washington 98195Keith L. AdamsDepartment of Biology, Indiana University, Bloomington, Indiana 47405Jeffrey D. PalmerDepartment of Biology, Indiana University, Bloomington, Indiana 47405Nga T. LaoDepartment of Genetics, Smurfit Institute, University of Dublin, Trinity College, Dublin 2, IrelandLaura HeggieDepartment of Genetics, Smurfit Institute, University of Dublin, Trinity College, Dublin 2, IrelandTony A. KavanaghDepartment of Genetics, Smurfit Institute, University of Dublin, Trinity College, Dublin 2, IrelandJulian M. HibberdDepartment of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, United KingdomJohn C. GrayDepartment of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, United KingdomClifford W. MordenDepartment of Botany, University of Hawaii, Honolulu, Hawaii 96816Patrick J. CalieDepartment of Biological Sciences, Eastern Kentucky University, Richmond, Kentucky 40475-3124Lars S. JermiinAustralian Genomic Information Centre and School of Biological Sciences, University of Sydney, Sydney, NSW 2006, AustraliaKenneth H. WolfeAustralian Genomic Information Centre and School of Biological Sciences, University of Sydney, Sydney, NSW 2006, Australia

2001en

ABI

Аннотация

We used DNA sequencing and gel blot surveys to assess the integrity of the chloroplast gene infA, which codes for translation initiation factor 1, in >300 diverse angiosperms. Whereas most angiosperms appear to contain an intact chloroplast infA gene, the gene has repeatedly become defunct in approximately 24 separate lineages of angiosperms, including almost all rosid species. In four species in which chloroplast infA is defunct, transferred and expressed copies of the gene were found in the nucleus, complete with putative chloroplast transit peptide sequences. The transit peptide sequences of the nuclear infA genes from soybean and Arabidopsis were shown to be functional by their ability to target green fluorescent protein to chloroplasts in vivo. Phylogenetic analysis of infA sequences and assessment of transit peptide homology indicate that the four nuclear infA genes are probably derived from four independent gene transfers from chloroplast to nuclear DNA during angiosperm evolution. Considering this and the many separate losses of infA from chloroplast DNA, the gene has probably been transferred many more times, making infA by far the most mobile chloroplast gene known in plants.

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Идентификаторы

DOI: 10.1105/tpc.13.3.645

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Цитирований: 2Использованных источников: 0

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