Статья

High-Sensitivity Detection of Carbohydrate Antigen 15-3 Using a Gold/Zinc Oxide Thin Film Surface Plasmon Resonance-Based Biosensor

Chia‐Chen ChangInstitute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan, Department of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, and Department of Laboratory Medicine, National Taiwan University Hospital, Taipei 10002, TaiwanNan‐Fu ChiuInstitute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan, Department of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, and Department of Laboratory Medicine, National Taiwan University Hospital, Taipei 10002, TaiwanDavid Shenhsiung LinInstitute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan, Department of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, and Department of Laboratory Medicine, National Taiwan University Hospital, Taipei 10002, TaiwanYu Chu-SuInstitute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan, Department of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, and Department of Laboratory Medicine, National Taiwan University Hospital, Taipei 10002, TaiwanYang-Hung LiangInstitute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan, Department of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, and Department of Laboratory Medicine, National Taiwan University Hospital, Taipei 10002, TaiwanChii‐Wann LinInstitute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan, Department of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, and Department of Laboratory Medicine, National Taiwan University Hospital, Taipei 10002, Taiwan

2010en

ABI

Аннотация

We report that gold/zinc oxide (Au/ZnO) nanocomposite films were effectively employed to enhance the performance of surface plasmon resonance (SPR) for the detection of tumor markers. Carbohydrate antigen 15.3 (CA15-3), a tumor marker for breast cancer, was chosen as a model analyte. We analyzed intensity response to the samples at various concentrations (0.0125 U/mL to 160 U/mL) in pleural fluid to evaluate the detection capability of the SPR biosensor based on Au/ZnO thin films. The linear range extended from 1 to 40 U/mL with a correlation coefficient of R(2) = 0.991 and a limit of detection reaching 0.025 U/mL at a signal-to-noise ratio of 3:1. Compared with the degree of the shift in SPR intensity induced by the specific binding event between antibody and antigen, the change of intensity on the Au/ZnO layers was increased by at least 2 fold over that on the gold/chromium (Au/Cr) layers. In addition, we determined that the Au/ZnO layers allowed for a detection limit 4 times lower than the Au/Cr layers, which are in widespread use as the sensing interfaces in current SPR-based detectors. In conclusion, the use of Au/ZnO films greatly enhanced the SPR signal yield for this bimolecular interaction and showed high sensitivity.

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Идентификаторы

DOI: 10.1021/ac901797j

Цитирования и источники

Цитирований: 2Использованных источников: 0

Показатели — AkademScholar