Статья

GhABF2, a bZIP transcription factor, confers drought and salinity tolerance in cotton (Gossypium hirsutum L.)

Chengzhen LiangBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaZhaohong MengBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaZhigang MengBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaWaqas MalikBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaRong YanBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKhin Myat LwinBiotechnology Research Department, Ministry of Science and Technology, Naypyidaw, MyanmarFazhuang LinBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaYuan WangBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaGuoqing SunBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaTao ZhouBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaΤao ZhuBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaJianying LiNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaShuangxia JinNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaSandui GuoBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaRui ZhangBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

2016en

ABI

Аннотация

The bZIP transcription factor (TF) act as an important regulator for the abscisic acid (ABA) mediated abiotic stresses signaling pathways in plants. Here, we reported the cloning and characterization of GhABF2, encoding for typical cotton bZIP TF. Overexpression of GhABF2 significantly improved drought and salt stress tolerance both in Arabidopsis and cotton. However, silencing of GhABF2 made transgenic cotton sensitive to PEG osmotic and salt stress. Expression of GhABF2 was induced by drought and ABA treatments but repressed by high salinity. Transcriptome analysis indicated that GhABF2 increases drought and salt tolerance by regulating genes related to ABA, drought and salt response. The proline contents, activity of superoxide dismutase (SOD) and catalase (CAT) were also significantly increased in GhABF2-overexpression cottons in comparison to wild type after drought and salt treatment. Further, an increase in fiber yield under drought and saline-alkali wetland exhibited the important role of GhABF2 in enhancing the drought and salt tolerance in transgenic lines. In conclusion, manipulation of GhABF2 by biotechnological tools could be a sustainable strategy to deploy drought and salt tolerance in cotton.

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Идентификаторы

DOI: 10.1038/srep35040

Цитирования и источники

Цитирований: 4Использованных источников: 0

Показатели — AkademScholar