Статья

Wet NH<sub>3</sub>-Triggered NH<sub>2</sub>-MIL-125(Ti) Structural Switch for Visible Fluorescence Immunoassay Impregnated on Paper

Shuzhen LvKey Laboratory of Analytical Science for Food Safety and Biology (MOE & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350116, People’s Republic of ChinaYun TangGrinnell College, 1115 Eighth Avenue, Grinnell, Iowa 50112, United StatesKangyao ZhangKey Laboratory of Analytical Science for Food Safety and Biology (MOE & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350116, People’s Republic of ChinaDianping TangKey Laboratory of Analytical Science for Food Safety and Biology (MOE & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350116, People’s Republic of China

2018en

ABI

Аннотация

This work has looked to explore an innovative and powerful visible fluorescence immunoassay method through wet NH3-triggered structural change of NH2-MIL-125(Ti) impregnated on paper for the detection of carcinoembryonic antigen (CEA). Gold nanoparticles heavily functionalized with glutamate dehydrogenase (GDH) and secondary antibody were used for generation of wet NH3 with a sandwiched immunoassay format. Paper-based analytical device (PAD) coated with NH2-MIL-125(Ti) exhibited good visible fluorescence intensity through wet NH3-triggeried structural change with high accuracy and reproducibility. Moreover, NH2-MIL-125(Ti)-based PAD displayed two visual modes of fluorescence color and physical color with the naked eye and allowed the detection of CEA at a concentration as low as 0.041 ng mL–1. Importantly, the PAD-based assay provides promise for use in the mass production of miniaturized devices and opens new opportunities for protein diagnostics and biosecurity.

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Идентификаторы

DOI: 10.1021/acs.analchem.8b04981

Цитирования и источники

Цитирований: 4Использованных источников: 0

Показатели — AkademScholar