Статья

Isolation and Characterization of Plant Growth Promoting Endophytic Bacteria from Desert Plants and Their Application as Bioinoculants for Sustainable Agriculture

Muneera D. F. AlKahtaniBiology Department, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh POX 102275-11675, Saudi ArabiaAmr FoudaBotany and Microbiology Department, Faculty of Science, AL-Azhar University, Nasr City, Cairo 11884, EgyptKotb A. AttiaCenter of Excellence in Biotechnology Research, King Saud University, Riyadh POX 2455-11451, Saudi ArabiaFahad AlotaibiBiodiversity Centre, Institut de Recherche en Biologie Végétale, Université de Montréal and Jardin botanique de Montréal, Montréal, QC 22001, CanadaAhmed M. EidBotany and Microbiology Department, Faculty of Science, AL-Azhar University, Nasr City, Cairo 11884, EgyptEmad A. EwaisBotany and Microbiology Department, Faculty of Science, AL-Azhar University, Nasr City, Cairo 11884, EgyptMohamed HijriAgroBioSciences, Mohammed VI Polytechnic University (UM6P), 43150 Ben Guerir, MoroccoMarc St‐ArnaudBiodiversity Centre, Institut de Recherche en Biologie Végétale, Université de Montréal and Jardin botanique de Montréal, Montréal, QC 22001, CanadaSaad El‐Din HassanBotany and Microbiology Department, Faculty of Science, AL-Azhar University, Nasr City, Cairo 11884, EgyptNaeem KhanDepartment of Agronomy, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USAYaser HafezExcellence Center (EPCRS), Plant Pathology and Biotechnology Laboratory, Faculty of Agriculture, Kafrelsheikh University, Kafr El Sheikh 33516, EgyptKhaled AbdelaalExcellence Center (EPCRS), Plant Pathology and Biotechnology Laboratory, Faculty of Agriculture, Kafrelsheikh University, Kafr El Sheikh 33516, Egypt

2020en

ABI

Аннотация

Desert plants are able to survive under harsh environmental stresses inherent to arid and semiarid regions due to their association with bacterial endophytes. However, the identity, functions, and the factors that influence the association of bacterial endophytes with desert plants are poorly known. These bacterial endophytes can be used as an untapped resource to favor plant growth and development in agro-ecosystems of arid regions. The present study is therefore focused on the isolation and identification of bacterial endophytes from two native medicinal plants (Fagonia mollis Delile and Achillea fragrantissima (Forssk) Sch. Bip.) growing spontaneously in the arid region of the South Sinai (Egypt), and characterization of their plant growth promoting (PGP) traits. Thirteen putative bacterial endophytes were isolated from the leaves of both plant species and characterized for their plant growth promoting abilities using molecular and biochemical approaches, as well as greenhouse trials. Selected endophytic bacterial strains were applied to maize plants (Zea mays L. var. Single cross Pioneer 30K08) to further evaluate their PGP abilities under greenhouse conditions. Isolated bacterial strains have variable plant growth promoting activities. Among these activities, isolated bacterial endophytes have the efficacy of phosphate solubilizing with clear zones ranging from 7.6 ± 0.3 to 9.6 ± 0.3 mm. Additionally, the obtained bacterial endophytes increased the productivity of indole acetic acid (IAA) in broth media from 10 to 60 µg·mL−1 with increasing tryptophan concentration from 1 to 5 mg·mL−1. Bacillus and Brevibacillus strains were frequently isolated from the leaves of both plant species, and had significant positive effects on plant growth and shoot phosphorus (P) and nitrogen (N) contents. Results suggest that these endophytes are good candidates as plant growth promoting inoculants to help reduce chemical input in conventional agricultural practices and increase nutrient uptake and stress resilience in plant species.

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Идентификаторы

DOI: 10.3390/agronomy10091325

Цитирования и источники

Цитирований: 3Использованных источников: 0

Показатели — AkademScholar