Статья

MiR‐130b suppresses prostate cancer metastasis through down‐regulation of MMP2

Qin ChenDepartment of Biochemistry and Molecular Cell Biology Shanghai Key Laboratory of Tumor Microenvironment and Inflammation Shanghai Jiao Tong University School of Medicine Shanghai ChinaXian ZhaoDepartment of Biochemistry and Molecular Cell Biology Shanghai Key Laboratory of Tumor Microenvironment and Inflammation Shanghai Jiao Tong University School of Medicine Shanghai ChinaHailong ZhangDepartment of Biochemistry and Molecular Cell Biology Shanghai Key Laboratory of Tumor Microenvironment and Inflammation Shanghai Jiao Tong University School of Medicine Shanghai ChinaHaihua YuanDepartment of Biochemistry and Molecular Cell Biology Shanghai Key Laboratory of Tumor Microenvironment and Inflammation Shanghai Jiao Tong University School of Medicine Shanghai ChinaMiaojun ZhuDepartment of Biochemistry and Molecular Cell Biology Shanghai Key Laboratory of Tumor Microenvironment and Inflammation Shanghai Jiao Tong University School of Medicine Shanghai ChinaQian SunDepartment of Biochemistry and Molecular Cell Biology Shanghai Key Laboratory of Tumor Microenvironment and Inflammation Shanghai Jiao Tong University School of Medicine Shanghai ChinaXueping LaiDepartment of Biochemistry and Molecular Cell Biology Shanghai Key Laboratory of Tumor Microenvironment and Inflammation Shanghai Jiao Tong University School of Medicine Shanghai ChinaYanli WangDepartment of Biochemistry and Molecular Cell Biology Shanghai Key Laboratory of Tumor Microenvironment and Inflammation Shanghai Jiao Tong University School of Medicine Shanghai ChinaJian HuangDepartment of Biochemistry and Molecular Cell Biology Shanghai Key Laboratory of Tumor Microenvironment and Inflammation Shanghai Jiao Tong University School of Medicine Shanghai ChinaJianshe YanShanghai Institute of Immunology Shanghai Jiao Tong University School of Medicine Shanghai ChinaJianxiu YuDepartment of Biochemistry and Molecular Cell Biology Shanghai Key Laboratory of Tumor Microenvironment and Inflammation Shanghai Jiao Tong University School of Medicine Shanghai China

2014en

ABI

Аннотация

Prostate cancer (PCa) is the most prevalent malignant carcinoma among males in western countries. Currently no treatments can cure advanced prostate cancers, so new diagnostic and therapeutic strategies are in urgent need. At present limited knowledge is available concerning the roles of dysregulated microRNAs in prostate cancer metastasis. In this study, we found that the expression of miR-130b was significantly down-regulated in prostate cancer cell lines and clinical prostate cancer tissues. Enforced over-expression of miR-130b in prostate cancer cells suppressed whereas knock-down of miR-130b increased cell migration and invasion. Using mouse model, we revealed that miR-130b-expressed prostate cancer cells displayed significant reduction in tumor metastasis. Furthermore, we identified and validated matrix metalloproteinase-2 (MMP2) as a direct target of miR-130b. Ectopic expression of MMP2 rescued miR-130b-suppressed cell migration and invasion, and knock-down of MMP2 antagonized the effect of silencing miR-130b.Taken together, our data reveal for the first time that miR-130b exerts a suppressive effect in prostate cancer metastasis through down-regulation of MMP2.

Перевод пока недоступен

Идентификаторы

DOI: 10.1002/mc.22204

Цитирования и источники

Цитирований: 2Использованных источников: 0

Показатели — AkademScholar