Статья

Rapid and Quantitative Detection of Prostate Specific Antigen with a Quantum Dot Nanobeads-Based Immunochromatography Test Strip

Xue LiInstitute of Nanobiotechnology, School of Materials Science and Engineering, School of Precision Instruments and Opto-Engineering, Tianjin University and Tianjin Key Laboratory of Composites and Functional Materials, Tianjin 300072, P.R. ChinaWenbin LiCancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100021, P.R. ChinaQiuhua YangInstitute of Nanobiotechnology, School of Materials Science and Engineering, School of Precision Instruments and Opto-Engineering, Tianjin University and Tianjin Key Laboratory of Composites and Functional Materials, Tianjin 300072, P.R. ChinaXiaoqun GongSchool of Life Science, Tianjin University, Tianjin 300072, P.R. ChinaWeisheng GuoInstitute of Nanobiotechnology, School of Materials Science and Engineering, School of Precision Instruments and Opto-Engineering, Tianjin University and Tianjin Key Laboratory of Composites and Functional Materials, Tianjin 300072, P.R. ChinaChunhong DongInstitute of Nanobiotechnology, School of Materials Science and Engineering, School of Precision Instruments and Opto-Engineering, Tianjin University and Tianjin Key Laboratory of Composites and Functional Materials, Tianjin 300072, P.R. ChinaJunqing LiuInstitute of Nanobiotechnology, School of Materials Science and Engineering, School of Precision Instruments and Opto-Engineering, Tianjin University and Tianjin Key Laboratory of Composites and Functional Materials, Tianjin 300072, P.R. ChinaLixue XuanCancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100021, P.R. ChinaJin ChangInstitute of Nanobiotechnology, School of Materials Science and Engineering, School of Precision Instruments and Opto-Engineering, Tianjin University and Tianjin Key Laboratory of Composites and Functional Materials, Tianjin 300072, P.R. China

2014en

ABI

Аннотация

Convenient and fast testing using an immunochromatography test strip (ICTS) enables rapid yes/no decisions regarding a disease to be made. However, the fundamental limitations of an ICTS, such as a lack of quantitative and sensitive analysis, severely hampers its application in reliable medical testing for the early detection of cancer. Herein, we overcame these limitations by integrating an ICTS with quantum dot nanobeads (QD nanobeads), which were fabricated by encapsulating QDs within modified poly(tert-butyl acrylate-co-ethyl acrylate-co-methacrylic acid) and served as a robust signal-generating reagent for the ICTS. Prostate specific antigen (PSA) was used as a model analyte to demonstrate the performance of the QD nanobeads-based ICTS platform. Under optimized conditions, the concentration of PSA could be determined within 15 min with high sensitivity and specificity using only 40 μL of sample. The detection limit was enhanced by ∼12-fold compared with that of an ICTS that used QDs encapsulated by commercial 11-mercaptoundecanoic acid (QDs@MUA) as the signal-generating reagent. At the same time, the possible clinical utility of this approach was demonstrated by measurements recorded from PSA-positive patient specimens. Our data suggest that the QD nanobeads-based ICTS platform is not only rapid and low-cost but also highly sensitive and specific for use in quantitative point-of-care diagnostics; thus, it holds promise for becoming a part of routine medical testing for the early cancer of detection.

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Идентификаторы

DOI: 10.1021/am5012782

Цитирования и источники

Цитирований: 2Использованных источников: 0

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