Статья

Bone Marrow Mesenchymal Stem Cell (BMSC) Exosomes Inhibit Angiogenesis and Enhance Autophagy Through Decreasing miR-21 in Cervical Cancer

Kewei ChenDepartments of Gynaecology and Obstetrics, Tongji Hospital of Tongji University, Shanghai, 200065, ChinaYiqin OuyangDepartments of Gynaecology and Obstetrics, Tongji Hospital of Tongji University, Shanghai, 200065, ChinaJunhua LiangDepartments of Gynaecology and Obstetrics, Tongji Hospital of Tongji University, Shanghai, 200065, ChinaHuaifang LiDepartments of Gynaecology and Obstetrics, Tongji Hospital of Tongji University, Shanghai, 200065, ChinaXiaowen TongDepartments of Gynaecology and Obstetrics, Tongji Hospital of Tongji University, Shanghai, 200065, ChinaYang� YangDepartments of Gynaecology and Obstetrics, Tongji Hospital of Tongji University, Shanghai, 200065, China

2021en

ABI

Аннотация

We aimed to explore the mechanism underlying the role of miR-21 derived from bone marrow mesenchymal stem cell exosomes (BMSC-exos) in cervical cancer (CC) and the relation between angiogenesis and autophagy. In this study, BMSC-exos were co-cultured with CC stem cells followed by analysis of miR-21 expression by RT-qPCR, autophagy after hunger-induced feeding by Acridine Orange fluorescent staining, angiogenesis by tube formation assay. Co-culture of BMSC-exos effectively reduced miR-21 expression in CC stem cells and enhanced autophagy as demonstrated by upregulated Beclin1 and LC3B with assembly of autophagosome ( p < 0.05), but the autophagy restored later. Moreover, in the presence of BMSC-exos, CC stem cell angiogenesis was suppressed by 79%. In conclusion, BMSC-exos enhance autophagy and inhibit angiogenesis in CC through decreasing miR-21, which provides a novel insight into etiology of CC.

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Идентификаторы

DOI: 10.1166/jbt.2021.2837

Цитирования и источники

Цитирований: 2Использованных источников: 0

Показатели — AkademScholar