Статья

Reporter Molecules Embedded Au@Ag Core-Shell Nanospheres as SERS Nanotags for Cardiac Troponin I Detection

Ding WangDepartment of Critical Care Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, ChinaYiru ZhaoSchool of Materials and Chemistry, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShen ZhangDepartment of Critical Care Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, ChinaLiping BaoSchool of Materials and Chemistry, University of Shanghai for Science and Technology, Shanghai 200093, ChinaHuijun LiSchool of Materials and Chemistry, University of Shanghai for Science and Technology, Shanghai 200093, ChinaJingcheng XuSchool of Materials and Chemistry, University of Shanghai for Science and Technology, Shanghai 200093, ChinaBin HeDepartment of Critical Care Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, ChinaXumin HouDepartment of Critical Care Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China

2022en

ABI

Аннотация

Rapid and accurate detection of acute myocardial infarction can improve patients’ chances of survival. Cardiac troponin I (cTn I) is an important diagnostic biomarker for acute myocardial infarction. However, current immunoassays are insufficient to accurately measure cTn I, as they have limited detection sensitivity and are time-consuming. Surface-enhanced Raman scattering (SERS) is a brilliant fingerprints diagnostic technique characterised by ultrasensitivity, fast response, and qualitative and quantitative analysis capabilities. In this study, reporter molecules (4-Mercaptobenzoic acid, 4-MBA) embedded Au@Ag core-shell nanospheres as SERS nanotags were prepared for the detection of cTn I. As the Raman reporters were embedded between the core and the shell, they could be protected from the external environment and nanoparticle aggregation. Excellent SERS performances were obtained due to the enhanced local electromagnetic field in the gap of core and shell metals. In a standard phosphate buffered saline (PBS) environment, the limit of detection for cTn I was 0.0086 ng mL−1 (8.6 ppt) with a good linear relationship. The excellent Raman detection performance was attributed to the localized surface plasmon resonance effect and strong electromagnetic field enhancement effect produced by the gap between the Au core and the Ag shell. The SERS nanotags we prepared were facile to synthesize, and the analysis procedure could be completed quickly (15 min), which made the detection of cTn I faster. Therefore, the proposed SERS nanotags have significant potential to be a faster and more accurate tool for acute myocardial infarction diagnostics.

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Идентификаторы

DOI: 10.3390/bios12121108

Цитирования и источники

Цитирований: 2Использованных источников: 0

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