Статья

Genetic Analysis of Human Norovirus Strains in Japan in 2016–2017

Koo NagasawaDepartment of Pediatrics, Graduate School of Medicine, Chiba University, Chiba, JapanYuki MatsushimaDivision of Virology, Kawasaki City Institute for Public Health, Kawasaki, JapanTakumi MotoyaDivision of Virology, Ibaraki Prefectural Institute of Public Health, Mito, JapanFuminori MizukoshiDepartment of Microbiology, Tochigi Prefectural Institute of Public Health and Environmental Science, Utsunomiya, JapanYo UekiNaomi SakonDepartment of Microbiology, Osaka Institute of Public Health, Osaka, JapanKoichi MurakamiInfectious Disease Surveillance Center, National Institute of Infectious Diseases, Musashimurayama, JapanTomomi ShimizuDivision of Virology, Kawasaki City Institute for Public Health, Kawasaki, JapanNobuhiko OkabeDivision of Virology, Kawasaki City Institute for Public Health, Kawasaki, JapanNoriko NagataDivision of Virology, Ibaraki Prefectural Institute of Public Health, Mito, JapanKomei ShirabeHiroto ShinomiyaDepartment of Microbiology, Ehime Prefectural Institute of Public Health and Environmental Science, Matsuyama, JapanWataru SuzukiEiken Chemical Co., Ltd., Biochemical Research Laboratory I Department-I, Shimotsuga, JapanMakoto KurodaPathogen Genomics Center, National Institute of Infectious Diseases, Shinjuku, JapanTsuyoshi SekizukaPathogen Genomics Center, National Institute of Infectious Diseases, Shinjuku, JapanYoshiyuki SuzukiDivision of Biological Science, Department of Information and Basic Science, Graduate School of Natural Sciences, Nagoya City University, Nagoya, JapanAkihide RyoDepartment of Microbiology, Yokohama City University School of Medicine, Yokohama, JapanKiyotaka FujitaSchool of Medical Technology, Faculty of Health Science, Gunma Paz University, Takasaki, JapanKazunori OishiInfectious Disease Surveillance Center, National Institute of Infectious Diseases, Musashimurayama, JapanKazuhiko KatayamaLaboratory of Viral Infection I, Kitasato Institute for Life Sciences, Graduate School of Infection Control Sciences, Kitasato University, Minato, JapanHirokazu KimuraDepartment of Microbiology, Yokohama City University School of Medicine, Yokohama, Japan

2018en

ABI

Аннотация

In the 2016/17 winter season in Japan, HuNoV GII.P16-GII.2 strains (2016 strains) emerged and caused large outbreaks of acute gastroenteritis. To better understand the outbreaks, we examined the molecular evolution of the VP1 gene and RdRp region in 2016 strains from patients by studying their time-scale evolutionary phylogeny, positive/negative selection, conformational epitopes, and phylodynamics. The time-scale phylogeny suggested that the common ancestors of the 2016 strains VP1 gene and RdRp region diverged in 2006 and 1999, respectively, and that the 2016 strain was the progeny of a pre-2016 GII.2. The evolutionary rates of the VP1 gene and RdRp region were around 10−3 substitutions/site/year. Amino acid substitutions (position 341) in an epitope in the P2 domain of 2016 strains were not found in pre-2016 GII.2 strains. Bayesian skyline plot analyses showed that the effective population size of the VP1 gene in GII.2 strains was almost constant for those 50 years, although the number of patients with NoV GII.2 increased in 2016. The 2016 strain may be involved in future outbreaks in Japan and elsewhere.

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Идентификаторы

DOI: 10.3389/fmicb.2018.00001

Цитирования и источники

Цитирований: 2Использованных источников: 0

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