Determination of the molecular mass of hydrolyzed fibroin obtained from natural silk fibroin by spectrophotometry

In our previous scientific publications, we have recommended spectrophotometry as a method for determining the molecular mass of silk fibroin. As a result of our research, we were able to obtain hydrolyzed fibroin (“HF”) with high polyfunctional high sorption properties from natural silk fibroin by thermal methods under acidic conditions and the influence of VHF rays. We used spectrophotometry to determine the molecular mass of “HF” obtained by two different methods. It was observed that there are differences between the molecular masses of “HF” obtained by thermal methods and under the influence of VHF rays. It was found that the molecular mass of “HF” obtained thermally under acidic conditions was 246,6 kDa, and the molecular mass of “HF” obtained under the influence of VHF rays was 307 kDa. The main reason for the different molecular masses of “HF” was considered to be the fact that the process duration of “HF” production methods varies.

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