Статья

Identification of existing pharmaceuticals and herbal medicines as inhibitors of SARS-CoV-2 infection

Jia-Tsrong JanGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;Ting-Jen Rachel ChengGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;Yu-Pu JuangSchool of Pharmacy, National Taiwan University, Taipei 110, Taiwan;Hsiu‐Hua MaGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;Ying‐Ta WuGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;Wen‐Bin YangGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;Cheng‐Wei ChengGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;Xiaorui ChenGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;Ting-Hung ChouInstitute of Chemistry, Academia Sinica, Taipei 128, Taiwan;Jiun‐Jie ShieInstitute of Chemistry, Academia Sinica, Taipei 128, Taiwan;Wei‐Chieh ChengGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;Rong‐Jie CheinInstitute of Chemistry, Academia Sinica, Taipei 128, Taiwan;Shi‐Shan MaoGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;Pi‐Hui LiangGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;Che MaGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;Shang‐Cheng HungGenomics Research Center, Academia Sinica, Taipei 115, Taiwan;Chi‐Huey WongDepartment of Chemistry, The Scripps Research Institute, La Jolla, CA 92037

2021en

ABI

Аннотация

Significance COVID-19 is a global pandemic currently lacking an effective cure. We used a cell-based infection assay to screen more than 3,000 agents used in humans and animals and identified 15 with antiinfective activity, ranging from 0.1 nM to 50 μM. We then used in vitro enzymatic assays combined with computer modeling to confirm the activity of those against the viral protease and RNA polymerase. In addition, several herbal medicines were found active in the cell-based infection assay. To further evaluate the efficacy of these promising compounds in animal models, we developed a challenge assay with hamsters and found that mefloquine, nelfinavir, and extracts of Ganoderma lucidum (RF3), Perilla frutescens , and Mentha haplocalyx were effective against SARS-CoV-2 infection.

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Идентификаторы

DOI: 10.1073/pnas.2021579118

Цитирования и источники

Цитирований: 3Использованных источников: 0

Показатели — AkademScholar