Статья

Imperatorin Attenuates the Proliferation of MCF-7 Cells in Combinationwith Radiotherapy or Hyperthermia

Masoud NajafiMedical Technology Research Center, Institute of Health Technology, Kermanshah University of Medical Sciences, Kermanshah, IranShahram TaebDepartment of Radiology, School of Paramedicine, Guilan University of Medical Sciences, Rasht, IranBagher FarhoodFaculty of Engineering and Natural Sciences, Sabanci University, Orta Mahalle, Üniversite Caddesi No. 27, Orhanlı, Tuzla, 34956 Istanbul, TurkeyPeyman AminiDepartment of Radiology, Faculty of Paramedical, Tehran University of Medical Sciences, Tehran, IranSaeedeh Jafari NodooshanDepartment of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, IranMilad AshrafizadehFaculty of Engineering and Natural Sciences, Sabanci University, Orta Mahalle, Üniversite Caddesi No. 27, Orhanlı, Tuzla, 34956 Istanbul, TurkeyAkbar AliasgharzadehDepartment of Medical Physics and Radiology, Faculty of Paramedical Sciences, Kashan University of Medical Sciences, Kashan, IranZarichehr VakiliDepartment of Pathology, School of Medicine, Kashan University of Medical Sciences, Kashan, IranSaeed TavakoliMedicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, IranTayebeh AryafarDepartment of Medical Physics and Biomedical Engineering, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, IranAhmed Eleojo MusaDepartment of Medical Physics and Biomedical Engineering, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran

2022en

ABI

Аннотация

BACKGROUND: Breast cancer is one of the most common types of malignancies in the world. Cancer resistance is an unavoidable consequence of therapy with radiation or other modalities. Ongoing research aims to improve cancer response to therapy. AIM: The aim of this study was to evaluate the possible sensitization effect of imperatorin (IMP) in combination with external radiotherapy (ERT) or HT. METHODS: After treatment of MCF-7 breast cancer cells with IMP, cells were exposed to 4 Gy X-rays or HT (42 °C for 1 hour). The viability of MCF-7 cells was measured using an MTT assay. Furthermore, the expression of pro-apoptotic genes, including Bax, Bcl-2, caspase-3, caspase-8, and caspase- 9, was investigated using real-time PCR. The sensitizing effect of IMP in combination with ERT or HT was calculated and compared to ERT or HT alone. RESULTS: Results showed an increase in the expression of pro-apoptotic genes and downregulation of anti-apoptotic Bcl-2 following ERT and HT. Furthermore, cell viability was reduced following these treatments. IMP was able to augment these effects of ERT and HT. CONCLUSION: IMP could increase the efficiency of HT and ERT. This effect of IMP may suggest it as an adjuvant for increasing the therapeutic efficiency of ERT.

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Идентификаторы

DOI: 10.2174/1874471015666220318122202

Цитирования и источники

Цитирований: 2Использованных источников: 0

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