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Aptasensing of ciprofloxacin residue using graphene oxide modified with gold nanoparticles and branched polyethyleneimine

Mansour MahmoudpourDepartment of Food Science and Technology, Faculty of Nutrition and Food Sciences, Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, IranJafar Ezzati Nazhad DolatabadiDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IranMohammad HasanzadehNutrition Research Center, Tabriz University of Medical Sciences, Tabriz, IranAziz Homayouni RadDepartment of Food Science and Technology, Faculty of Nutrition and Food Sciences, Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, IranMohammadali TorbatiDepartment of Food Science and Technology, Faculty of Nutrition and Food Sciences, Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, IranFarzad SeidiJiangsu Co-Innovation Center for Efficient Processing and Utilization of Forest Resources and International Innovation Center for Forest Chemicals and Materials, Nanjing Forestry University, Nanjing 210037, China
2022en
ABI

Аннотация

cyclic voltammetry techniques. The construction and morphology of the GO-PEI-AuNPs composite were evaluated by UV-Vis spectroscopy, transmission electron microscopy, and field emission scanning electron microscopy. Under optimal conditions, the suggested scaffold can offer an acceptable linear range of 0.001 to 100 μM and a low limit of quantification of 0.001 μM for selective and sensitive monitoring of CFX in real samples. The effectiveness of the apta-assay was confirmed by detection of CFX in pasteurized and local milk samples for which suitable analytical results were achieved. It is expected that the developed substrate can be facilely extended to other aptamer-based multiplex screening platforms in actual food and environmental samples.

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