Статья

Analysis of Renal Cell Carcinoma as a First Step for Developing Mass Spectrometry-Based Diagnostics

Kentaro YoshimuraDepartment of Anatomy and Cell Biology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, JapanLee Chuin ChenInterdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, JapanMridul Kanti MandalClean Energy Research Center, University of Yamanashi, Kofu, Yamanashi, JapanTadao NakazawaDepartment of Human Pathology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, JapanZhan YuCollege of Chemistry and Biology, Shenyang Normal University, Liaoning, ChinaTakahito UchiyamaInterdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, JapanHirokazu HoriInterdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, JapanKunio TanabeDepartment of Anatomy and Cell Biology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, JapanTakeo KubotaDepartment of Epigenetic Medicine, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, JapanHideki FujiiDepartment of Surgery, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, JapanRyohei KatohDepartment of Human Pathology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, JapanKenzo HiraokaClean Energy Research Center, University of Yamanashi, Kofu, Yamanashi, JapanSén TakedaDepartment of Anatomy and Cell Biology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, Japan

2012en

ABI

Аннотация

Immediate diagnosis of human specimen is an essential prerequisites in medical routines. This study aimed to establish a novel cancer diagnostics system based on probe electrospray ionization-mass spectrometry (PESI-MS) combined with statistical data processing. PESI-MS uses a very fine acupuncture needle as a probe for sampling as well as for ionization. To demonstrate the applicability of PESI-MS for cancer diagnosis, we analyzed nine cases of clear cell renal cell carcinoma (ccRCC) by PESI-MS and processed the data by principal components analysis (PCA). Our system successfully delineated the differences in lipid composition between non-cancerous and cancerous regions. In this case, triacylglycerol (TAG) was reproducibly detected in the cancerous tissue of nine different individuals, the result being consistent with well-known profiles of ccRCC. Moreover, this system enabled us to detect the boundaries of cancerous regions based on the expression of TAG. These results strongly suggest that PESI-MS will be applicable to cancer diagnosis, especially when the number of data is augmented.

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Идентификаторы

DOI: 10.1007/s13361-012-0447-2

Цитирования и источники

Цитирований: 3Использованных источников: 0

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