Обзорная статья

A Comprehensive Review of Fluorescence Correlation Spectroscopy

Lan YuSchool of Physics and Optoelectronic Engineering, Xidian University, Xi'an, China,Yunze LeiSchool of Physics and Optoelectronic Engineering, Xidian University, Xi'an, China,Ying MaSchool of Physics and Optoelectronic Engineering, Xidian University, Xi'an, China,Min LiuSchool of Physics and Optoelectronic Engineering, Xidian University, Xi'an, China,Juanjuan ZhengSchool of Physics and Optoelectronic Engineering, Xidian University, Xi'an, China,Dan DanState Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an, ChinaPeng GaoSchool of Physics and Optoelectronic Engineering, Xidian University, Xi'an, China,

2021en

ABI

Аннотация

Fluorescence correlation spectroscopy (FCS) is a powerful technique for quantification of molecular dynamics, and it has been widely applied in diverse fields, e.g. , biomedicine, biophysics, and chemistry. By time-correlation of the fluorescence fluctuations induced by molecules diffusing through a focused light, FCS can quantitatively evaluate the concentration, diffusion coefficient, and interaction of the molecules in vitro or in vivo . In this review, the basic principle and implementation of FCS are introduced. Then, the advances of FCS variants are reviewed, covering dual-color FCCS, multi-focus FCS, pair correlation function (pCF), scanning FCS, focus-reduced FCS, SPIM-FCS, and inverse-FCS. Besides, the applications of FCS are demonstrated with the measurement of local concentration, hydrodynamic radius, diffusion coefficient, and the interaction of different molecules. Lastly, a discussion is given by summarizing the pros and cons of different FCS techniques, as well as the outlooks and perspectives of FCS.

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Идентификаторы

DOI: 10.3389/fphy.2021.644450

Цитирования и источники

Цитирований: 3Использованных источников: 0

Показатели — AkademScholar