Статья

Cell-type-specific PtrWOX4a and PtrVCS2 form a regulatory nexus with a histone modification system for stem cambium development in Populus trichocarpa

Xiufang DaiState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaRui ZhaiState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaJiaojiao LinState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaZhifeng WangNational Key Laboratory of Plant Molecular Genetics and CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, ChinaDekai MengState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaMeng LiState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaYuli MaoState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaBoyuan GaoState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaHongyan MaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaBaofeng ZhangState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaYi SunState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaShuang LiState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaChenguang ZhouState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaYing‐Chung Jimmy LinDepartment of Life Sciences and Institute of Plant Biology, College of Life Science, National Taiwan University, Taipei, Taiwan, ChinaJack WangForest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC, USAVincent L. ChiangForest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC, USAWei LiState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [email protected]

2023en

ABI

Аннотация

Stem vascular cambium cells in forest trees produce wood for materials and energy. WOX4 affects the proliferation of such cells in Populus. Here we show that PtrWOX4a is the most highly expressed stem vascular-cambium-specific (VCS) gene in P. trichocarpa, and its expression is controlled by the product of the second most highly expressed VCS gene, PtrVCS2, encoding a zinc finger protein. PtrVCS2 binds to the PtrWOX4a promoter as part of a PtrWOX13a-PtrVCS2-PtrGCN5-1-PtrADA2b-3 protein tetramer. PtrVCS2 prevented the interaction between PtrGCN5-1 and PtrADA2b-3, resulting in H3K9, H3K14 and H3K27 hypoacetylation at the PtrWOX4a promoter, which led to fewer cambium cell layers. These effects on cambium cell proliferation were consistent across more than 20 sets of transgenic lines overexpressing individual genes, gene-edited mutants and RNA interference lines in P. trichocarpa. We propose that the tetramer-PtrWOX4a system may coordinate genetic and epigenetic regulation to maintain normal vascular cambium development for wood formation.

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Идентификаторы

DOI: 10.1038/s41477-022-01315-7

Цитирования и источники

Цитирований: 2Использованных источников: 0

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