Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles
Uta ErdbrüggerDivision of Nephrology University of Virginia Health System Charlottesville Virginia USACharles J. BlijdorpDepartment of Internal medicine Division of Nephrology and Transplantation Erasmus MC University Medical Center Rotterdam Rotterdam The NetherlandsIrene V. BijnsdorpDepartment of Urology Amsterdam UMC location VUmc Cancer Center Amsterdam Amsterdam The NetherlandsFrancesc E. BorràsREMAR‐IVECAT Group, “Germans Trias i Pujol” Health Science Research Institute and Nephrology Department “Germans Trias i Pujol” University Hospital Badalona SpainDylan BurgerKidney Research Centre Ottawa Hospital Research Institute and Department of Cellular and Molecular Medicine University of Ottawa CanadaBenedetta BussolatiDepartment of Molecular Biotechnology and Health Science University of Torino Torino ItalyJames Brian ByrdDepartment of Internal Medicine University of Michigan Medical School Ann Arbor Michigan USAAled ClaytonTissue Microenvironment Group Division of Cancer & Genetics School of Medicine Cardiff University United KingdomJames W. DearCentre for Cardiovascular Science Queen's Medical Research Institute University of Edinburgh Edinburgh United KingdomJuan Manuel Falcón‐PérezExosomes Laboratory and Metabolomics Platform Bizkaia Technology Park CIC bioGUNE‐BRTA Derio SpainCristina GrangeDepartment of Medical Sciences University of Torino Torino ItalyAndrew F. HillDepartment of Biochemistry and Genetics, La Trobe Institute for Molecular Science La Trobe University Bundoora Victoria AustraliaHarry HolthöferInstitute for Molecular Medicine Finland FIMM University of Helsinki Helsinki FinlandEwout J. HoornDepartment of Internal medicine Division of Nephrology and Transplantation Erasmus MC University Medical Center Rotterdam Rotterdam The NetherlandsGuido JensterErasmus MC Cancer Institute Department of Urology Laboratory of Experimental Urology University Medical Center Rotterdam Erasmus MC Rotterdam The NetherlandsConnie R. JiménezDepartment of Urology Amsterdam UMC location VUmc Cancer Center Amsterdam Amsterdam The NetherlandsKerstin JunkerDepartment of Urology Saarland University Medical Centre Homburg GermanyJohn B. KleinDepartment of Medicine University of Louisville School of Medicine Louisville Kentucky USAMark A. KnepperEpithelial Systems Biology Laboratory Systems Biology Center National Heart, Lung, and Blood Institute National Institutes of Health Bethesda Maryland USAErik H. KoritzinskyRenal Diagnostics and Therapeutics Unit National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health Bethesda Maryland USAJames M. LutherDivision of Clinical Pharmacology Department of Medicine Vanderbilt University Medical Center Nashville Tennessee USAMetka LenassiInstitute of Biochemistry Faculty of Medicine University of Ljubljana Ljubljana SloveniaJanne LeivoDepartment of Biochemistry University of Turku Turku FinlandInge MertensCentre for Proteomics, University of Antwerp and Health Unit, VITO Antwerp BelgiumLuca MusanteDivision of Nephrology University of Virginia Health System Charlottesville Virginia USAEline OeyenCentre for Proteomics, University of Antwerp and Health Unit, VITO Antwerp BelgiumMaija PuhkaInstitute for Molecular Medicine Finland FIMM University of Helsinki Helsinki FinlandMartin E. van RoyenDepartment of Pathology Erasmus MC Cancer Institute University Medical Center Rotterdam Erasmus MC Rotterdam The NetherlandsCatherine SánchezDepartment of Urology Clínica las Condes Research Core Santiago ChileCarolina SoekmadjiDepartment of Cell and Molecular Biology QIMR Berghofer Medical Research Institute Brisbane AustraliaVisith ThongboonkerdMedical Proteomics Unit Office for Research and Development Faculty of Medicine Siriraj Hospital Mahidol University Bangkok ThailandVolkert van SteijnDepartment of Chemical Engineering TU Delft Delft The NetherlandsGerald W. VerhaeghDepartment of Urology Radboud University Medical Center Nijmegen The NetherlandsJason WebberInstitute of Life Science 1 Swansea University Medical School Singleton Park Campus Swansea UKKenneth W. WitwerDepartments of Molecular and Comparative Pathobiology and Neurology John Hopkins University School of Medicine Baltimore Maryland USAPeter S.T. YuenRenal Diagnostics and Therapeutics Unit National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health Bethesda Maryland USALei ZhengDepartment of Laboratory Medicine Nanfang Hospital Southern Medical University Guangzhou Guangdong ChinaAlicia LlorenteDepartment for Mechanical Electronics and Chemical Engineering Oslo Metropolitan University Oslo NorwayElena S. Martens‐UzunovaErasmus MC Cancer Institute Department of Urology Laboratory of Experimental Urology University Medical Center Rotterdam Erasmus MC Rotterdam The Netherlands
2021en
ABI
Аннотация
Abstract Urine is commonly used for clinical diagnosis and biomedical research. The discovery of extracellular vesicles (EV) in urine opened a new fast‐growing scientific field. In the last decade urinary extracellular vesicles (uEVs) were shown to mirror molecular processes as well as physiological and pathological conditions in kidney, urothelial and prostate tissue. Therefore, several methods to isolate and characterize uEVs have been developed. However, methodological aspects of EV separation and analysis, including normalization of results, need further optimization and standardization to foster scientific advances in uEV research and a subsequent successful translation into clinical practice. This position paper is written by the Urine Task Force of the Rigor and Standardization Subcommittee of ISEV consisting of nephrologists, urologists, cardiologists and biologists with active experience in uEV research. Our aim is to present the state of the art and identify challenges and gaps in current uEV‐based analyses for clinical applications. Finally, recommendations for improved rigor, reproducibility and interoperability in uEV research are provided in order to facilitate advances in the field.
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