Статья

<i>OsTTG1</i>, a WD40 repeat gene, regulates anthocyanin biosynthesis in rice

Xinghai YangGuangxi Key Laboratory of Rice Genetics and Breeding Rice Research Institute Guangxi Academy of Agricultural Sciences Nanning 530007 ChinaJunrui WangGuangxi Key Laboratory for Polysaccharide Materials and Modifications School of Marine Sciences and Biotechnology Guangxi University for Nationalities Nanning 530007 ChinaXiuzhong XiaGuangxi Key Laboratory of Rice Genetics and Breeding Rice Research Institute Guangxi Academy of Agricultural Sciences Nanning 530007 ChinaZongqiong ZhangGuangxi Key Laboratory of Rice Genetics and Breeding Rice Research Institute Guangxi Academy of Agricultural Sciences Nanning 530007 ChinaJie HeAgro‐products Quality Safety and Testing Technology Research Institute Guangxi Academy of Agricultural Sciences Nanning 530007 ChinaBaoxuan NongGuangxi Key Laboratory of Rice Genetics and Breeding Rice Research Institute Guangxi Academy of Agricultural Sciences Nanning 530007 ChinaLuo TongPingGuangxi Key Laboratory of Rice Genetics and Breeding Rice Research Institute Guangxi Academy of Agricultural Sciences Nanning 530007 ChinaRui FengGuangxi Key Laboratory of Rice Genetics and Breeding Rice Research Institute Guangxi Academy of Agricultural Sciences Nanning 530007 ChinaYanyan WuBiotechnology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007 ChinaYinghua PanGuangxi Key Laboratory of Rice Genetics and Breeding Rice Research Institute Guangxi Academy of Agricultural Sciences Nanning 530007 ChinaFaqian XiongCash Crops Research Institute Guangxi Academy of Agricultural Sciences Nanning 530007 ChinaYu ZengGuangxi Key Laboratory of Rice Genetics and Breeding Rice Research Institute Guangxi Academy of Agricultural Sciences Nanning 530007 ChinaCan ChenGuangxi Key Laboratory of Rice Genetics and Breeding Rice Research Institute Guangxi Academy of Agricultural Sciences Nanning 530007 ChinaHui GuoGuangxi Key Laboratory of Rice Genetics and Breeding Rice Research Institute Guangxi Academy of Agricultural Sciences Nanning 530007 ChinaZhijian XuGuangxi Key Laboratory of Rice Genetics and Breeding Rice Research Institute Guangxi Academy of Agricultural Sciences Nanning 530007 ChinaDanting LiGuangxi Key Laboratory of Rice Genetics and Breeding Rice Research Institute Guangxi Academy of Agricultural Sciences Nanning 530007 ChinaGuofu DengGuangxi Key Laboratory of Rice Genetics and Breeding Rice Research Institute Guangxi Academy of Agricultural Sciences Nanning 530007 China

2021en

ABI

Аннотация

Anthocyanins play an important role in the growth of plants, and are beneficial to human health. In plants, the MYB-bHLH-WD40 (MBW) complex activates the genes for anthocyanin biosynthesis. However, in rice, the WD40 regulators remain to be conclusively identified. Here, a crucial anthocyanin biosynthesis gene was fine mapped to a 43.4-kb genomic region on chromosome 2, and a WD40 gene OsTTG1 (Oryza sativa TRANSPARENT TESTA GLABRA1) was identified as ideal candidate gene. Subsequently, a homozygous mutant (osttg1) generated by CRISPR/Cas9 showed significantly decreased anthocyanin accumulation in various rice organs. OsTTG1 was highly expressed in various rice tissues after germination, and it was affected by light and temperature. OsTTG1 protein was localized to the nucleus, and can physically interact with Kala4, OsC1, OsDFR and Rc. Furthermore, a total of 59 hub transcription factor genes might affect rice anthocyanin biosynthesis, and LOC_Os01g28680 and LOC_Os02g32430 could have functional redundancy with OsTTG1. Phylogenetic analysis indicated that directional selection has driven the evolutionary divergence of the indica and japonica OsTTG1 alleles. Our results suggest that OsTTG1 is a vital regulator of anthocyanin biosynthesis, and an important gene resource for the genetic engineering of anthocyanin biosynthesis in rice and other plants.

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Идентификаторы

DOI: 10.1111/tpj.15285

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Цитирований: 2Использованных источников: 0

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