Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells
Shutoku MatsuyamaDepartment of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan;Naganori NaoDepartment of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan;Kazuya ShiratoDepartment of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan;Miyuki KawaseDepartment of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan;Shinji SaitoInfluenza Virus Research Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan;Ikuyo TakayamaInfluenza Virus Research Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan;Noriyo NagataDepartment of Pathology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan;Tsuyoshi SekizukaPathogen Genomics Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan;Hiroshi KatohDepartment of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan;Fumihiro KatoDepartment of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan;Masafumi SakataDepartment of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan;Maino TaharaDepartment of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan;Satoshi KutsunaDisease Control and Prevention Center, National Center for Global Health and Medicine, Tokyo 162-8655, JapanNorio OhmagariDisease Control and Prevention Center, National Center for Global Health and Medicine, Tokyo 162-8655, JapanMakoto KurodaPathogen Genomics Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan;Tadaki SuzukiDepartment of Pathology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan;Tsutomu KageyamaInfluenza Virus Research Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan;Makoto TakedaDepartment of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan;
2020en
ABI
Аннотация
A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2.
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