Artificial Ectomycorrhiza Formation on Roots of Air-Layered Pinus densiflora Saplings by Inoculation with Lyophyllum shimeji

Saplings of Pinus densiflora were grown using an air-layering procedure which produced roots without mycorrhizal infection. The plants successfully formed ectomycorrhizal roots when planted in clay pots containing inocula of Lyophyllum shimeji mycelia. Light and electron microscope observation of the mycorrhizae showed a hyphal mantle covering the root surface, Hartig nets in the intercellular spaces of the root cortex, and starch grains accumulated in the cells of the vascular cylinder of the roots. Mycelia isolated from the mycorrhizae and incubated for about two mo in pure culture formed fruiting bodies of L. shimeji. Ten months after inoculation, fruiting bodies of L. shimeji formed in a pot with an air layered of P. densiflora sapling.

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