Статья

Insect Gut Bacterial Diversity Determined by Environmental Habitat, Diet, Developmental Stage, and Phylogeny of Host

Ji‐Hyun YunDepartment of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul, South KoreaSeong Woon RohDepartment of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul, South KoreaTae Woong WhonDepartment of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul, South KoreaMi‐Ja JungDepartment of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul, South KoreaMin‐Soo KimDepartment of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul, South KoreaDoo‐Sang ParkBiological Resource Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon, South KoreaChangmann YoonJeju Center, Korea Basic Science Institute, Jeju, South KoreaYoung‐Do NamDepartment of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul, South KoreaYun‐Ji KimDepartment of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul, South KoreaJung‐Hye ChoiDepartment of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul, South KoreaJoon Yong KimDepartment of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul, South KoreaNa‐Ri ShinDepartment of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul, South KoreaSunghee KimSchool of Biological Science, Seoul National University, and National Creative Research Initiative Center for Symbiosystem, Seoul, South KoreaWon‐Jae LeeSchool of Biological Science, Seoul National University, and National Creative Research Initiative Center for Symbiosystem, Seoul, South KoreaJin‐Woo BaeDepartment of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul, South Korea

2014en

ABI

Аннотация

Insects are the most abundant animals on Earth, and the microbiota within their guts play important roles by engaging in beneficial and pathological interactions with these hosts. In this study, we comprehensively characterized insect-associated gut bacteria of 305 individuals belonging to 218 species in 21 taxonomic orders, using 454 pyrosequencing of 16S rRNA genes. In total, 174,374 sequence reads were obtained, identifying 9,301 bacterial operational taxonomic units (OTUs) at the 3% distance level from all samples, with an average of 84.3 (± 97.7) OTUs per sample. The insect gut microbiota were dominated by Proteobacteria (62.1% of the total reads, including 14.1% Wolbachia sequences) and Firmicutes (20.7%). Significant differences were found in the relative abundances of anaerobes in insects and were classified according to the criteria of host environmental habitat, diet, developmental stage, and phylogeny. Gut bacterial diversity was significantly higher in omnivorous insects than in stenophagous (carnivorous and herbivorous) insects. This insect-order-spanning investigation of the gut microbiota provides insights into the relationships between insects and their gut bacterial communities.

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Идентификаторы

DOI: 10.1128/aem.01226-14

Цитирования и источники

Цитирований: 2Использованных источников: 0

Показатели — AkademScholar