Spectrophotometric Analysis of Chlorophylls and Carotenoids from Commonly Grown Fern Species by Using Various Extracting Solvents

Present investigation is performed on the comparative extraction of photosynthetic pigments (chlorophyll-a, chlorophyll-b and carotenoids) by using solvents of different chemical nature. The study is also concern on the extraction ratio of biomolecules with respect to time duration/variation. Different trend is observed in extraction rate for chlorophylls and carotenoids. Highest extraction of chlorophylls (Ch-a and Ch-b) is noted for DEE (except chlorophyll b in Adiantum sp.). Whereas maximum extraction of caroteniods is performed by acetone in Adiantum sp., and for Crystiella sp. and Drypteris sp DMSO execute best extraction of carotenoids. Significant variations (p<0.01) in pigment concentrations are also noted for sampled species exposed to different chemical extractant solvents for different time duration.

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