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Observation of Nine Types of Spinach Pollen Morphology by Scanning Electron Microscopy

Meng MengState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaZhiyuan LiuState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaZhaosheng XuState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaHelong ZhangState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaHongbing SheState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaWei QianState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
2024en
ABI

Аннотация

Spinach (Spinacia oleracea L.) is a dioecious, wind-pollinated, and important green leafy vegetable that is widely cultivated worldwide. The characteristics of spinach pollen remain unclear. Herein, we investigated the pollen microscopic morphology of nine spinach genotypes by scanning electron microscopy (SEM). Because pollen grains of spinach are isodiametric and spheroidal, the following data for the pollen properties were observed: the maximum diameter of the pollen (27.66–33.05 um), pore diameter max (1.26–2.57 um), the number of visible pores (30–44), the density of pore elements/100 μm2 (4.33–11.00), and the density of spinule elements/10 μm2 (4.00–5.33). These are useful characteristics in distinguishing the species. Furthermore, by applying the cluster analysis method, based on key components, nine varieties are categorized into two main classes. These results provide a useful reference for the classification and identification of different spinach varieties.

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