Anti‑inflammatory and anti‑rheumatic activities <i>in vitro</i> of alkaloids separated from <i>Aconitum soongoricum Stapf</i>

The aim of the present study was to investigate the cell proliferation‑inhibiting and anti‑rheumatic activities of chemical components from <em>Aconitum soongoricum Stapf</em>. Chemical constituents of <em>Aconitum soongoricum Stapf</em>. were separated and purified by silica gel and Sephadex LH‑20 chromatography. Structure was identified by spectroscopic technique, and physical/chemical properties were analyzed. The following four compounds were identified: i) Aconitine, ii) songorine, iii) 16, 17‑dihydro‑12β, 16β‑epoxynapelline, and iv) 12‑epi‑napelline. Cell Counting kit‑8 assay was performed to assess cell proliferation. ELISA was conducted to determine the cytokine contents, and reverse transcription‑quantitative polymerase chain reaction and Western blot analysis were performed to detect the mRNA and protein expression levels. Compared with the lipopolysaccharide (LPS) group, the contents of IL‑6, IL‑1β, TNF‑α and PGE‑2 in the culture supernatant were significantly declined in the leflunomide + LPS and intervention+LPS groups, as well as the mRNA expression levels of HIF‑1α, VEGFA and TLR4. Treatments with songorine, benzoylaconine and aconitine (at different concentrations) significantly inhibited the proliferation of HFLS‑RA cells. Compared with the LPS group, the contents of PGE‑2, IL‑6, IL‑1β and TNF‑α in the culture supernatant were significantly decreased in the intervention groups, and the mRNA expression levels of TLR4, HIF‑1α and VEGFA in the cells in the intervention groups. Songorine, benzoylaconine and aconitine from <em>Aconitum soongoricum Stapf</em>. have anti‑rheumatic activities <em>in vitro</em>, which may inhibit the proliferation of HFLS‑RA cells, and the underlying mechanisms may be associated with inhibiting the inflammatory cytokine production and downregulating the expression levels of HIF‑1α, VEGF and TLR4.

Перевод пока недоступен