PHYSICOCHEMICAL PROPERTIES OF CHITIN AND CHITOSAN FROM DIED HONEY BEES APIS MELLIFERA OF UZBEKISTAN

This article presents the results of obtaining biopolymers of chitin and chitosan from Uzbekistan a new promising source- honeybee bodies Apis Mellifera . To obtain chitin, at the first stage, the protein (deproteination) and mineral (demineralization) constituents of subpestilence, i.e. put them in soluble state and removed. Demineralization was carried out with 2 M hudrochloric acid for 5 hours at room temperature. The process of deproteination is carried out by processing the crushed raw materials with 1 N sodium hudroxide solution for 1 hour at a temperature of 800С. Each process was accompanied by washing the raw materials to a natural wash water reaction (pH=7). At the next stage, chitin deacetylation under the influence of a 35% aqueous NaOH solution at a temperature of 850С for 4 hours produced chitosan, which is a high molecular weight glucosamine polymer, and dried at 50-550С. The resulting mass was decolorized with a 3% H2O2 solution and washed ethanol.

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