Статья

Silver Nanoparticles Synthesized by Using Bacillus cereus SZT1 Ameliorated the Damage of Bacterial Leaf Blight Pathogen in Rice

Temoor AhmedDepartment of Bioinformatics and Biotechnology, Government College University, Faisalabad 38000, PakistanMuhammad ShahidDepartment of Bioinformatics and Biotechnology, Government College University, Faisalabad 38000, PakistanMuhammad NomanState Key Laboratory of Rice BiologyMuhammad Bilal Khan NiaziSchool of Chemical and Materials Engineering (SCME), National University of Sciences & Technology (NUST), Sector H-12, Islamabad 44000, PakistanFaisal MahmoodDepartment of Environmental Science and Engineering, Government College University, Faisalabad 38000, PakistanIrfan ManzoorDepartment of Bioinformatics and Biotechnology, Government College University, Faisalabad 38000, PakistanYang ZhangState Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaBin LiState Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaYong YangState Key Laboratory for Quality and Safety of Agro-Products, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaChengqi YanInstitute of Plant Virology, Ningbo University, Ningbo 315211, ChinaJianping ChenInstitute of Plant Virology, Ningbo University, Ningbo 315211, China

2020en

ABI

Аннотация

Amongst serious biotic factors deteriorating crop yield, the most destructive pathogen of rice is Xanthomonas oryzae pv. oryzae (Xoo), which causes bacterial leaf blight (BLB) disease. This study involved targeted use of biogenic silver nanoparticles (AgNPs) to control BLB in order to cope with the disadvantages of chemical disease control. AgNPs were biologically synthesized from natively isolated Bacillus cereus strain SZT1, which was identified through 16S rRNA gene sequence analysis. Synthesis of AgNPs in bacterial culture supernatant was confirmed through UV-VIS spectroscopy. Fourier transform infrared spectroscopy (FTIR) confirmed that the existence of AgNPs was stabilized with proteins and alcoholic groups. X-ray diffraction (XRD) data revealed the crystalline nature and imaging with scanning electron microscopy (SEM) and transmission electron microscopy (TEM), showing the spherical shape of AgNPs with particle sizes ranging from 18 to 39 nm. The silver presence in AgNPs was further confirmed by energy dispersive spectra. Biogenic AgNPs showed substantial antibacterial activity (24.21 ± 1.01 mm) for Xoo. In a pot experiment, AgNPs were found to be effective weapons for BLB by significantly increasing the plant biomass with a decreased cellular concentration of reactive oxygen species and increased concentration of antioxidant enzyme activity.

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Идентификаторы

DOI: 10.3390/pathogens9030160

Цитирования и источники

Цитирований: 2Использованных источников: 0

Показатели — AkademScholar